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protein phosphatase 1 regulatory subunit 12A; myosin phosphatase-targeting subunit 1; myosin phosphatase target subunit 1; protein phosphatase myosin-binding subunit (PPP1R12A, MBS, MYPT1)
Function:
- regulatory subunit of protein phosphatase 1 (PP1)
- regulates myosin phosphatase activity
- phosphorylated by CIT (Rho-associated kinase)
- phosphorylated cooperatively by ROCK1 & CDC42BP on Thr-696
- phosphorylated on upon DNA damage, probably by ATM or ATR
- in vitro, phosphorylation of Ser-695 by PKA & PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1
- interacts with ARHA & CIT (putative)
- binds PPP1R12B, ROCK1 & IL16
- interacts directly with PRKG1. non-covalent dimer of 2 dimers; PRKG1-PRKG1 & PPP1R12A-PPP1R12A
Structure:
- heterotetramerization is mediated by interaction between a coiled-coil of PRKG1 & the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of myosin phosphatase (putative)
- contains 6 ANK repeats
Compartment:
- cytoplasm
- long actomyosin filaments & stress fibers
Alternative splicing: named isoforms=4
General
ankyrin repeat domain protein (ANKRD)
protein phosphatase-1 regulatory subunit
phosphoprotein
Properties
COMPARTMENT: cytoplasm
INHIBITS: phosphatase
SIZE: entity length = 1030 aa
MW = 115 kD
MOTIF: ankyrin repeat
NAME: ankyrin repeat
SITE: 39-68
ankyrin repeat
NAME: ankyrin repeat
SITE: 72-101
ankyrin repeat
NAME: ankyrin repeat
SITE: 105-134
ankyrin repeat
NAME: ankyrin repeat
SITE: 138-164
ankyrin repeat
NAME: ankyrin repeat
SITE: 198-227
ankyrin repeat
NAME: ankyrin repeat
SITE: 231-260
Ser phosphorylation site {S299}
Ser phosphorylation site {S422}
Thr phosphorylation site {T443}
Ser phosphorylation site {S445}
Tyr phosphorylation site {Y446}
Ser phosphorylation site {S473}
Ser phosphorylation site {S477}
Ser phosphorylation site {S507}
Thr phosphorylation site {T508}
Ser phosphorylation site {S509}
threonine-rich region {519-660}
MOTIF: threonine residue (SEVERAL)
Ser phosphorylation site {S566}
Ser phosphorylation site {S569}
Ser phosphorylation site {S668}
Ser phosphorylation site {S692}
Ser phosphorylation site {S695}
Thr phosphorylation site {T696}
lysine-rich region {721-753}
MOTIF: lysine residue (SEVERAL)
serine-rich region {773-795}
MOTIF: serine residue (SEVERAL)
Ser phosphorylation site {S852}
Ser phosphorylation site {S862}
Ser phosphorylation site {S871}
Tyr phosphorylation site {Y890}
Tyr phosphorylation site {Y901}
Ser phosphorylation site {S910}
Tyr phosphorylation site {Y911}
Database Correlations
OMIM 602021
UniProt O14974
Pfam PF00023
Entrez Gene 4659
Kegg hsa:4659
References
UniProt :accession O14974