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multiplex polymerase chain reaction (PCR) assay

Indications: - bacterial meningitis: multiplex PCR assay can detect 14 pathogens in 1 hour [1] - BioFire FilmArray Pneumonia Panel (BioFire PN) targets 33 genes from viruses. bacteria & antibiotic-resistance genes [2] Clinical significance: - Biofire PN detects an average of 1.2 bacterial pathogens in specimens [2] - standard bacterial culture detected only 0.5 organisms per sample - Hemophilus influenzae in 33% - Streptococcus pneumoniae in 20% - Staphylococcus aureus in 20% - gram-negative bacilli in 18% - Moraxella catarrhalis in 12% - Biofire PN detects viral pathogens in 51% of specimens [2]

General

polymerase chain reaction (PCR) laboratory panel

References

  1. Medical Knowledge Self Assessment Program (MKSAP) 14, 15, 16, 17, 18, 19. American College of Physicians, Philadelphia 2006, 2009, 2012, 2015, 2018, 2021. - Medical Knowledge Self Assessment Program (MKSAP) 19 Board Basics. An Enhancement to MKSAP19. American College of Physicians, Philadelphia 2022
  2. Falsey AR et al. Real-life assessment of BioFire FilmArray pneumonia panel in adults hospitalized with respiratory illness. J Infect Dis 2023 Jun 27; [e-pub]. PMID: 37369370 https://academic.oup.com/jid/advance-article/doi/10.1093/infdis/jiad221/7209038