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Msx2-interacting protein (SPEN homolog, SMART/HDAC1-associated repressor protein, SPEN, KIAA0929, MINT, SHARP)

Function: - corepressor protein - regulates key pathways, including Notch pathway - negative regulator of Notch pathway via its interaction with RBPSUH, which prevents association of NOTCH1 with RBPSUH, thius suppresses transactivation activity of Notch signaling - blocks differentiation of precursor B-cells into marginal zone B-cells - represses transcription via recruitment of large complexes containing histone deacetylase proteins. - may bind both to DNA & RNA - interacts with MSX2 - interacts with NCOR2, HDAC1, HDAC2, RBBP4, MBD3 & MTA1L1 - interacts with RBPSUH; interaction may prevent interaction of RBPSUH with NOTCH1 - interacts with nuclear receptors RAR & PPARD - interacts with RAR in absence of ligand - bind to steroid receptor RNA coactivator SRA - phosphorylated upon DNA damage, probably by ATM or ATR Structure: - belongs to the RRM Spen family - contains 1 RID (receptor interacting) domain, mediates interaction with nuclear receptors - contains 4 RRM domain (RNA recognition motif) - contains 1 SPOC domain, mediates interaction with NCOR2, essential for repressive activity Compartment: nucleus, associates with chromatin. Alternative splicing: named isoforms=1 Expression: - expressed in brain, testis, spleen, thymus > kidney, liver, mammary gland, skin - induced by hormone 17-beta-estradiol (E2)

General

phosphoprotein transcriptional silencer (transcriptional repressor)

Properties

SIZE: MW = 402 kD entity length = 3664 aa COMPARTMENT: cell nucleus MOTIF: DNA-binding motif SITE: 1-573 MOTIF: RNP motif NAME: RNP motif SITE: 6-81 FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif NAME: ribonucleoprotein-1 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif ribonucleoprotein-2 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif arginine-rich region {125-277} MOTIF: arginine residue (SEVERAL) serine-rich region {240-325} MOTIF: serine residue (SEVERAL) Ser phosphorylation site {S260} Ser phosphorylation site {S263} Thr phosphorylation site {T266} RNP motif NAME: RNP motif SITE: 335-415 FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif NAME: ribonucleoprotein-1 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif ribonucleoprotein-2 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif RNP motif NAME: RNP motif SITE: 438-513 FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif NAME: ribonucleoprotein-1 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif ribonucleoprotein-2 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif RNP motif NAME: RNP motif SITE: 517-589 FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif NAME: ribonucleoprotein-1 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif ribonucleoprotein-2 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif arginine-rich region {616-810} MOTIF: arginine residue (SEVERAL) coiled coil {688-715} Ser phosphorylation site {S725} Ser phosphorylation site {S727} Ser phosphorylation site {S736} Ser phosphorylation site {S740} coiled coil {977-1004} coiled coil {1170-1191} Ser phosphorylation site {S1261} Ser phosphorylation site {S1268} Ser phosphorylation site {S1278} Ser phosphorylation site {S1283} Ser phosphorylation site {S1380} Ser phosphorylation site {S1382} coiled coil {1408-1428} coiled coil {1496-1529} coiled coil {1592-1612} Ser phosphorylation site {S1797} Ser phosphorylation site {S1857} coiled coil {1928-1944} Ser phosphorylation site {S2101} Ser phosphorylation site {S2120} MSX2 interaction {2130-2464} RID {2201-2707} MOTIF: Thr phosphorylation site {T2393} proline-rich region SITE: 2428-2520 MOTIF: proline residue (SEVERAL) RBPSUH interaction {2709-2870} proline-rich region SITE: 3220-3482 MOTIF: proline residue (SEVERAL) SPOC {3498-3664}

Database Correlations

UniProt Q96T58 PFAM correlations

References

UniProt :accession Q96T58