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MRN complex
Function:
1) double strand break repair complex
2) cellular response to DNA damage
3) maintenance of chromosome integrity
4) DNA double-strand break repair
5) DNA recombination
6) maintenance of telomere integrity
7) cell cycle checkpoint control
8) meiosis
9) single-strand endonuclease activity (MRE11A)
10) double-strand-specific 3'-5' exonuclease activity (MRE11A)
11) DNA-binding, holding ends in proximity (RAD50)
12) nibrin modulates DNA damage signal sensing recruits ATM, ATR, DNA-PKcs to the DNA damage sites
13) interacts with Artemis protein
Structure:
- consists of at least:
a) MRE11A (2)
b) RAD50 (2)
c) Nibrin
Pathology:
1) see individual subnits (MRE11A, RAD50, Nibrin)
2) complex is inactivated by infection with adenovirus E4 & degraded by viral oncoproteins
General
molecular complex
Properties
SUBUNITS: double-strand break repair protein MRE11A (2)
COMPARTMENT: cell nucleus
MOTIF: Thr phosphorylation site {T74}
histidine residue {H129}
Ser phosphorylation site {S649}
Ser phosphorylation site {S678}
Ser phosphorylation site {S688}
Ser phosphorylation site {S689}
DNA repair protein RAD50 (2)
COMPARTMENT: cell nucleus
MOTIF: ATP-binding site
NAME: ATP-binding site
SITE: 36-43
coiled coil {228-359}
MOTIF: Ser phosphorylation site {S237}
coiled coil {401-598}
Zinc-hook {635-734}
MOTIF: coiled coil {635-673}
Ser phosphorylation site {S635}
Ser phosphorylation site {S640}
Zn+2-binding site
SITE: 681-681
Zn+2-binding site
SITE: 684-684
Thr phosphorylation site {T690}
coiled coil {706-734}
coiled coil {789-1079}
nibrin
MOTIF: forkhead-associated (FHA) domain {24-83}
BRCA1 C-terminal (BRCT) motif
SITE: 105-181
Ser phosphorylation site {S278}
Thr phosphorylation site {T337}
Ser phosphorylation site {S341}
Ser phosphorylation site {S343}
Ser phosphorylation site {S397}
Ser phosphorylation site {S432}
glutamine-rich region {448-451}
MOTIF: glutamine residue (SEVERAL)
nuclear translocation signal {461-467}
Thr phosphorylation site {T602}
Ser phosphorylation site {S615}
EEXXXDDL {736-743}
References
UniProt :accession Q96SD1, O60934
Components
DNA repair protein RAD50; hRAD50 (RAD50)
double-strand break repair protein MRE11A; meiotic recombination 11 homolog A; MRE11 homolog A; meiotic recombination 11 homolog 1; MRE11 homolog 1 (MRE11A, HNGS1, MRE11)
nibrin; Nijmegen breakage syndrome protein 1; cell cycle regulatory protein p95 (NBN, NBS, NBS1, P95)