MAP kinase-interacting serine/threonine kinase 2 (MAP kinase signal-integrating kinase 2, MKNK2, GPRK7 MNK2)

Function: - may play a role in the response to environmental stress & cytokines - appears to regulate transcription by phosphorylating EIF4E, thus increasing its affinity for the 7-methylguanosine- containing mRNA cap - activated by dual phosphorylation of Thr-244 & Thr-249 &/or by phosphorylation of Thr-379 - interacts with the C-terminal regions of EIF4G1 & EIF4G2 - also binds to dephosphorylated ERK1 & ERK2 - isoform 2 interacts with ESR2 ATP + a protein ADP + a phosphoprotein Cofactor: Mg⁺², binds 1 Zn⁺² per subunit Structure: - monomer - belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family - contains 1 protein kinase domain Alternative splicing: named isoforms=5 Expression: - ubiquitously expressed in all tissues examined - isoform 2 is expressed at higher levels in the ovary than is isoform 1

Properties

SIZE: entity length = 465 aa MW = 52 kD STATE: active state MOTIF: kinase domain SITE: 84-388 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 90-98 ATP-binding site NAME: ATP-binding site SITE: 113-113 aspartate residue {D205} Thr phosphorylation site {T244} Thr phosphorylation site {T249} Zn+2-binding site SITE: 299-299 Zn+2-binding site SITE: 311-311 Zn+2-binding site SITE: 314-314 Thr phosphorylation site {T379}

Database Correlations

OMIM 605069 UniProt Q9HBH9 Pfam PF00069 Kegg hsa:2872 ENZYME 2.7.11.1

References

UniProt :accession Q9HBH9