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matrix metalloproteinase-7 (matrixin-7, matrin, urine metalloproteinase, pump-1 protease, matrylisin, MMP7, MPSL1, PUMP1)

Function: - degrades casein, gelatins of types 1, 3, 4, & 5, & fibronectin - activates procollagenase cleavage of 14-Ala-|-Leu-15 & 16-Tyr-|-Leu-17 in B chain of insulin - no action on collagen types 1, 2, 4, 5 - cleaves gelatin chain alpha-2(I) > alpha-1(I) Cofactor: - binds 2 Ca+2 per subunit - binds 2 Zn+2 per subunit Structure: - conserved Cys present in the cysteine-switch motif binds the catalytic Zn+2, thus inhibiting the enzyme; dissociation of the Cys from the Zn+2 upon the activation-peptide release activates the enzyme - belongs to the peptidase M10A family

General

matrixin or matrix metalloproteinase

Properties

SIZE: entity length = 267 aa MW = 30 kD COMPARTMENT: extracellular matrix MOTIF: signal sequence {1-17} Cysteine switch {85-92} MOTIF: Zn+2-binding site SITE: 87-87 Ca+2-binding site SITE: 153-153 Zn+2-binding site SITE: 163-163 Zn+2-binding site SITE: 165-165 Ca+2-binding site SITE: 170-170 Ca+2-binding site SITE: 171-171 Ca+2-binding site SITE: 173-173 Ca+2-binding site SITE: 175-175 Zn+2-binding site SITE: 178-178 Ca+2-binding site SITE: 185-185 Ca+2-binding site SITE: 187-187 Ca+2-binding site SITE: 189-189 Zn+2-binding site SITE: 191-191 Ca+2-binding site SITE: 193-193 Ca+2-binding site SITE: 196-196 Zn+2-binding site SITE: 214-214 glutamate residue {E215} Zn+2-binding site SITE: 218-218 Zn+2-binding site SITE: 224-224

Database Correlations

OMIM 178990 UniProt P09237 PFAM correlations Entrez Gene 4316 KEGG correlations ENZYME 3.4.24.23

References

UniProt :accession P09237