matrix metalloproteinase-13 (matrixin-13, collagenase-3, MMP13)

Function: - degrades collagen type 1 - does not act on gelatin or casein - could have a role in tumoral process Cofactor: - binds 4 Ca⁺² per subunit - binds 2 Zn⁺² per subunit Structure: - conserved Cys present in the cysteine-switch motif binds the catalytic Zn⁺², thus inhibiting the enzyme; dissociation of the Cys from the Zn⁺² upon the activation-peptide release activates the enzyme - belongs to the peptidase M10A family - contains 4 hemopexin-like domains Expression: seems to be specific to breast carcinomas Pathology: - defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia type 2

General

collagenase matrixin or matrix metalloproteinase

Properties

SIZE: entity length = 471 aa MW = 54 kD COMPARTMENT: extracellular matrix MOTIF: signal sequence {1-19} Cysteine switch {94-101} MOTIF: Zn+2-binding site SITE: 96-96 N-glycosylation site {N117} N-glycosylation site {N152} Ca+2-binding site SITE: 162-162 Zn+2-binding site SITE: 172-172 Zn+2-binding site SITE: 174-174 Ca+2-binding site SITE: 179-179 Ca+2-binding site SITE: 180-180 Zn+2-binding site SITE: 187-187 Ca+2-binding site SITE: 194-194 Ca+2-binding site SITE: 196-196 Ca+2-binding site SITE: 198-198 Zn+2-binding site SITE: 200-200 Ca+2-binding site SITE: 205-205 Zn+2-binding site SITE: 222-222 glutamate residue {E223} Zn+2-binding site SITE: 226-226 Zn+2-binding site SITE: 232-232 cysteine residue {C284} MODIFICATION: cysteine residue {C471} Hemopexin-like 1 {290-332} MOTIF: Ca+2-binding site SITE: 291-291 Ca+2-binding site SITE: 293-293 Hemopexin-like 2 {334-377} MOTIF: Ca+2-binding site SITE: 335-335 Ca+2-binding site SITE: 337-337 Hemopexin-like 3 {382-429} MOTIF: Ca+2-binding site SITE: 383-383 Ca+2-binding site SITE: 385-385 Hemopexin-like 4 {431-471} MOTIF: Ca+2-binding site SITE: 432-432 Ca+2-binding site SITE: 434-434 cysteine residue {C471} MODIFICATION: cysteine residue {C284}

Database Correlations

OMIM correlations MORBIDMAP 600108 UniProt P45452 PFAM correlations Entrez Gene 4322 Kegg hsa:4322