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centromere protein F; CENP-F; AH antigen; kinetochore protein CENPF; mitosin (CENPF)
Function:
- required for kinetochore function & chromosome segregation in mitosis
- required for kinetochore localization of dynein, LIS1, NDE1 & NDEL1
- regulates recycling of the plasma membrane by acting as a link between recycling vesicles & the microtubule network though its association with STX4 & SNAP25
- acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating activity of RB protein during cell division & proliferation
- may play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle & in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes
- interaction with RB directs embryonic stem cells toward a cardiac lineage
- involved in the regulation of DNA synthesis & hence cell cycle progression, via its C-terminus
- potential role regulating skeletal myogenesis & in cell differentiation in embryogenesis
- role in dendritic cell regulation of T-cell immunity against Chlamydia
- hyperphosphorylated during mitosis
- phosphorylated upon DNA damage, probably by ATM or ATR
- interacts with & STX4 (via C-terminus) (putative)
- interacts (via N-terminus) with RBL1, RBL2 & SNAP25
- self-associates
- interacts with CENP-E & BUBR1 (via C-terminus)
- interacts (via C-terminus) with NDE1, NDEL1 & RB1
Structure:
- belongs to the centromere protein F family
Compartment:
- cytoplasm, cytoskeleton, spindle
- perinuclear region, nuclear matrix
- chromosome, centromere, kinetochore
- relocalizes to the kinetochore/centromere (coronal surface of the outer plate) & the spindle during mitosis
- observed in nucleus during interphase
- not in the nucleolus during interphase
- at metaphase becomes localized to the kinetochore & mitotic apparatus as well as cytoplasm
- by telophase, is concentrated within the intracellular bridge at either side of the mid-body
Expression:
- gradually accumulates during the cell cycle, reaching peak levels in G2 & M phase, & is rapidly degraded upon completion of mitosis
Laboratory:
- centromere protein F Ab in serum
General
centromere protein
phosphoprotein
Properties
SIZE: entity length = 3210 aa
MW = 368 kD
COMPARTMENT: cytoplasm
cell nucleus
MOTIF: domain {1-481}
MOTIF: coiled coil {14-197}
Ser phosphorylation site {S106}
Thr phosphorylation site {T144}
Thr phosphorylation site {T154}
Thr phosphorylation site {T253}
Ser phosphorylation site {S268}
coiled coil {273-769}
MOTIF: Ser phosphorylation site {S274}
Ser phosphorylation site {S276}
Ser phosphorylation site {S769}
Ser phosphorylation site {S821}
coiled coil {823-1328}
MOTIF: Ser phosphorylation site {S834}
Ser phosphorylation site {S1248}
Ser phosphorylation site {S1255}
Ser phosphorylation site {S1282}
Tyr phosphorylation site {Y1315}
Thr phosphorylation site {T1319}
Ser phosphorylation site {S1324}
consensus repeat {1435-1530}
consensus repeat {1531-1626}
MOTIF: Ser phosphorylation site {S1590}
coiled coil {1642-1746}
Ser phosphorylation site {S1747}
Ser phosphorylation site {S1748}
Ser phosphorylation site {S1750}
coiled coil {1862-2987}
MOTIF: Ser phosphorylation site {S1988}
NDE1/NDEL1 interaction {2122-2447}
consensus repeat {2207-2386}
consensus repeat {2389-2568}
centromere localization {2488-3113}
MOTIF: self-association {2488-2925}
Ser phosphorylation site {S2512}
Ser phosphorylation site {S2513}
Ser phosphorylation site {S2638}
nuclear localization {2927-3113}
Ser phosphorylation site {S2996}
Ser phosphorylation site {S3007}
nuclear translocation signal {3015-3032}
Ser phosphorylation site {S3094}
Ser phosphorylation site {S3119}
Ser phosphorylation site {S3122}
Ser phosphorylation site {S3150}
Ser phosphorylation site {S3175}
Ser phosphorylation site {S3179}
CaaX motif
SITE: C-TERMINUS
EFFECTOR-BOUND: farnesyl
Database Correlations
OMIM 600236
UniProt P49454
PFAM correlations
Entrez Gene 1063
Kegg hsa:1063
References
- UniProt :accession P49454
- Atlas of Genetics & Cytogenetics in Oncology & Haematology
http://atlasgeneticsoncology.org/genes/CENPFID40057ch1q41.html