E3 ubiquitin-protein ligase MID2; midin-2; midline defect 2; midline-2; RING finger protein 60; tripartite motif-containing protein 1 (MID2, FXY2, RNF60, TRIM1)

Function: - protein modification; protein ubiquitination - phosphorylated on Ser & Thr - homodimer or heterodimer with MID1 - interacts with IGBP1 Structure: - the tripartite motif (RBCC; RING- & B box-type Zn⁺² fingers & coiled coil domains) mediates dimerization - association with microtubules is dependent on the C-terminal B30.2 domain - belongs to the TRIM/RBCC family - contains 2 B box-type Zn⁺² fingers - contains 1 B30.2/SPRY domain - contains 1 COS domain - contains 1 fibronectin F3 module - contains 1 RING-type Zn⁺² finger Compartment: -cytoplasm, cytoskeleton; microtubule-associated Alternative splicing: named isoforms=2 Expression: - low level in fetal kidney & lung, & in adult prostate, ovary & small intestine Note: - uncertain whether Met-1 or Met-21 is the initiator

General

midline protein tripartite motif-containing protein (trim protein) E3 ubiquitin ligase; ubiquitin-ligating enzyme E3; N end-recognizing protein

Properties

SIZE: entity length = 735 aa MW = 83 kD COMPARTMENT: cytoplasm MOTIF: RING-finger {30-80} EFFECTOR-BOUND: Zn+2 FOR-BINDING-OF: DNA motif zinc finger B box SITE: 137-184 EFFECTOR-BOUND: Zn+2 zinc finger B box SITE: 190-232 EFFECTOR-BOUND: Zn+2 coiled coil {233-301} COS {340-399} fibronectin type III domain or F3 module {398-531} B30.2/SPRY {516-709}

Database Correlations

OMIM 300204 UniProt Q9UJV3 PFAM correlations Entrez Gene 11043 Kegg hsa:11043

References

UniProt :accession Q9UJV3