alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B; UDP-N-acetylglucosamine:alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVb; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVb; N-acetylglucosaminyltransferase IVb, GnT-IVb, GlcNAc-T IVb (MGAT4B)

Function: - glycosyltransferase - role in transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans - catalyzes formation of GlcNAcbeta1-4 branch on GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans - essential for the production of tri- & tetra-antennary N-linked sugar chains - has lower affinities for donors or acceptors than MGAT4A, thought not main contributor in N-glycan biosynthesis Cofactor: divalent metal cations Kinetic parameters: - KM=0.242 mM for UDP-GlcNAc Structure: belongs to the glycosyltransferase 54 family Compartment: Golgi Alternative splicing: named isoforms=2 Expression: widely expressed Pathology: - strongly overexpressed in pancreatic cancer

Properties

SIZE: MW = 63 kD entity length = 548 aa COMPARTMENT: golgi MOTIF: transmembrane domain {8-28} coiled coil {36-83} N-glycosylation site {N87} N-glycosylation site {N103}

Database Correlations

OMIM 604561 UniProt Q9UQ53 Pfam PF04666 Entrez Gene 11282 Kegg hsa:11282 ENZYME 2.4.1.145

References

UniProt :accession Q9UQ53