alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A; UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVa; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVa; N-acetylglucosaminyltransferase IVa, GnT-IVa, GlcNAc-T IVa, [contains: alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble form] (MGAT4A)

Function: - glycosyltransferase, glycosylation pathway - role in transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans - atalyzes the formation of GlcNAcbeta1-4 branch on GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans - required for production of tri- & tetra-antennary N-linked sugar chains - role in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thus controlling cell-surface expression of SLC2A2 in pancreatic beta cells UDP-N-acetyl-D-glucosamine + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D- mannosyl-R UDP + 3-(2,4-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D- mannosyl-R Cofactor: divalent metal cations Kinetic parameters: - KM=0.118 mM for UDP-GlcNAc Structure: belongs to the glycosyltransferase 54 family Compartment: - Golgi membrane, secreted - membrane & processed soluble form Expression: - expressed in pancreas, spleen, thymus, prostate, small intestine, peripheral blood leukocytes, lymph node Pathology: - strongly overexpressed in choriocarcinoma cancer cell lines - down-regulated in pancreatic cancer

Properties

SIZE: MW = 62 kD entity length = 535 aa COMPARTMENT: golgi MOTIF: transmembrane domain {5-27} coiled coil {28-63} N-glycosylation site {N77} N-glycosylation site {N458}

Database Correlations

OMIM 604623 UniProt Q9UM21 Pfam PF04666 Entrez Gene 11320 Kegg hsa:11320 ENZYME 2.4.1.145

References

UniProt :accession Q9UM21