MED8 mediator complex (mediator of RNA polymerase 2 transcription)

Function: 1) can either repress or activate transcription 2) essential for basal & regulated expression of nearly all RNA polymerase 2-dependent genes 3) may act as a bridge, conveying regulatory information from enhancers & other control elements to the promoter Subunits: - MED12/TRAP230, PPARBP/TRAP220, CRSP6/TRAP80, CDK8, CCNC, MED6, CRSP9/MED7, CRSP8/TRAP37, USP47/TRFP, NUT2/MED10, SOH1, THRAP6/TRAP25, MED21, MED28 & THRAP4/MED24

Properties

SUBUNITS: mediator of RNA polymerase II transcription subunit 12 COMPARTMENT: cell nucleus MOTIF: Tyr phosphorylation site {Y166} Ser phosphorylation site {S635} Ser phosphorylation site {S698} Ser phosphorylation site {S700} Ser phosphorylation site {S1258} Ser phosphorylation site {S1269} CTNNB1/GLI3 interaction {1616-2051} MOTIF: proline-rich region SITE: 1732-1777 MOTIF: proline residue (SEVERAL) glutamine-rich region {1900-2168} MOTIF: glutamine residue (SEVERAL) mediator of RNA polymerase II transcription subunit 1 COMPARTMENT: cell nucleus MOTIF: Mediator/THRA interaction {1-670} MOTIF: ESR1 interaction {16-590} Ser phosphorylation site {S18} Mediator interaction {108-212} Mediator interaction {215-390} THRA interaction {405-644} VDR interaction {542-789} MOTIF: LXXLL motif 1 {604-608} PPARGC1A/THRA interaction {622-701} GATA1 interaction {637-716} LXXLL motif 2 {645-649} ESR1 interaction {656-1066} MOTIF: Ser phosphorylation site {S664} Ser phosphorylation site {S770} Ser phosphorylation site {S771} Ser phosphorylation site {S772} Ser phosphorylation site {S774} Ser phosphorylation site {S795} Thr phosphorylation site {T805} Ser phosphorylation site {S953} Thr phosphorylation site {T1032} Ser phosphorylation site {S1049} Thr phosphorylation site {T1051} Thr phosphorylation site {T1057} serine-rich region {1078-1482} MOTIF: serine residue (SEVERAL) Ser phosphorylation site {S1134} Ser phosphorylation site {S1155} Ser phosphorylation site {S1207} Thr phosphorylation site {T1215} Ser phosphorylation site {S1223} Ser phosphorylation site {S1232} TP53 interaction {1249-1421} Ser phosphorylation site {S1252} Ser phosphorylation site {S1403} Ser phosphorylation site {S1433} Ser phosphorylation site {S1439} Thr phosphorylation site {T1440} Thr phosphorylation site {T1457} Ser phosphorylation site {S1463} Ser phosphorylation site {S1479} Ser phosphorylation site {S1481} Ser phosphorylation site {S1482} lysine-rich region {1496-1529} MOTIF: lysine residue (SEVERAL) mediator of RNA polymerase II transcription subunit 17 COMPARTMENT: cell nucleus MOTIF: Ser phosphorylation site {S37} Ser phosphorylation site {S50} Ser phosphorylation site {S53} Ser phosphorylation site {S55} Ser phosphorylation site {S75} Ser phosphorylation site {S76} Thr phosphorylation site {T143} Ser phosphorylation site {S185} cyclin-dependent kinase-8 STATE: active state MOTIF: kinase domain SITE: 21-335 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 27-35 ATP-binding site NAME: ATP-binding site SITE: 52-52 aspartate residue {D151} glutamine-rich region {373-377} MOTIF: glutamine residue (SEVERAL) cyclin C COMPARTMENT: cell nucleus CELL-CYCLE: G1/S MOTIF: ubiquitination site (destruction box, D-box) SITE: N-TERMINAL FOR-BINDING-OF: ubiquitin PEST region cyclin box FOR-BINDING-OF: cyclin-dependent kinase mediator of RNA polymerase II transcription subunit 6 COMPARTMENT: cell nucleus mediator of RNA polymerase II transcription subunit 7 COMPARTMENT: cell nucleus MOTIF: Ser phosphorylation site {S194} CRSP complex subunit 8 COMPARTMENT: cell nucleus ubiquitin carboxyl-terminal hydrolase 47 COMPARTMENT: cytoplasm MOTIF: cysteine residue {C197} histidine residue {H503} Ser phosphorylation site {S832} Tyr phosphorylation site {Y836} Ser phosphorylation site {S910} Thr phosphorylation site {T1015} Ser phosphorylation site {S1353} NUT2 mediator of RNA polymerase 2 transcription subunit 31 COMPARTMENT: cell nucleus MOTIF: glutamine-rich region {122-125} MOTIF: glutamine residue (SEVERAL) thyroid hormone receptor-associated protein 6 COMPARTMENT: cell nucleus MOTIF: coiled coil {70-94} coiled coil {133-173} RNA polymerase 2 holoenzyme component SRB7 COMPARTMENT: cell nucleus mediator of RNA polymerase 2 transcription subunit 28 COMPARTMENT: cytoplasm cell nucleus MOTIF: coiled coil {109-145} mediator of RNA polymerase II transcription subunit 24 COMPARTMENT: cell nucleus MOTIF: LXXLL motif 1 {128-132} LXXLL motif 2 {344-348} LXXLL motif 3 {448-452} LXXLL motif 4 {557-561} LXXLL motif 5 {788-792} LXXLL motif 6 {857-861} Ser phosphorylation site {S862} Ser phosphorylation site {S873}

References

UniProt :accession Q9Y5B9

Components

CRSP complex subunit 8; cofactor required for Sp1 transcriptional activation subunit 8; transcriptional coactivator; p37 TRAP/SMCC/PC2 subunit (CRSP8, CRSP34, MED27) cyclin C (CCNC, hSRB11) cyclin-dependent kinase-8 (CDK8, protein kinase K35, mediator complex subunit CDK8) mediator of RNA polymerase 2 transcription subunit 28; endothelial-derived protein 1; mediator complex subunit 28; Merlin & Grb2-interacting cytoskeletal protein; Magicin; tumor angiogenesis marker EG-1 (MED28, EG1, FKSG20) mediator of RNA polymerase 2 transcription subunit 31 (mediator complex subunit SOH1, hSOH1, MED31, SOH1, CGI-125) mediator of RNA polymerase II transcription subunit 12; activator-recruited cofactor 240 kD component; ARC240; CAG repeat protein 45; mediator complex subunit 12; OPA-containing protein; thyroid hormone receptor-associated protein complex 230 kD component; trap230; trinucleotide repeat-containing gene 11 protein (MED12, ARC240, CAGH45, HOPA, KIAA0192, TNRC11, TRAP230) mediator of RNA polymerase II transcription subunit 17; activator-recruited cofactor 77 kD component; ARC77; cofactor required for Sp1 transcriptional activation subunit 6; CRSP complex subunit 6; mediator complex subunit 17; thyroid hormone receptor-associated protein complex 80 kD component; trap80; transcriptional coactivator CRSP77; vitamin D3 receptor-interacting protein complex 80 kD component; DRIP80 (MED17, ARC77, CRSP6, DRIP77, DRIP80, TRAP80) mediator of RNA polymerase II transcription subunit 1; activator-recruited cofactor 205 kD component; ARC205; mediator complex subunit 1; peroxisome proliferator-activated receptor-binding protein; PBP; PPAR-binding protein; thyroid hormone receptor-associated protein complex 220 kD component; trap220; thyroid receptor-interacting protein 2; TR-interacting protein 2; TRIP-2; vitamin D receptor-interacting protein complex component DRIP205; p53 regulatory protein RB18A (MED1, ARC205, CRSP1, CRSP200, DRIP205, DRIP230, PBP, PPARBP, PPARGBP, RB18A, TRAP220, TRIP2) mediator of RNA polymerase II transcription subunit 24; activator-recruited cofactor 100 kD component; ARC100; cofactor required for Sp1 transcriptional activation subunit 4; CRSP complex subunit 4; mediator complex subunit 24; thyroid hormone receptor-associated protein 4; thyroid hormone receptor-associated protein complex 100 kD component; trap100; hTRAP100; vitamin D3 receptor-interacting protein complex 100 kD component; DRIP100 (MED24, ARC100, CRSP4, DRIP100, KIAA0130, THRAP4, TRAP100) mediator of RNA polymerase II transcription subunit 6; activator-recruited cofactor 33 kD component; ARC33; mediator complex subunit 6; hmed6; renal carcinoma antigen NY-REN-28 (MED6, ARC33) mediator of RNA polymerase II transcription subunit 7; hMED7; activator-recruited cofactor 34 kD component; ARC34; cofactor required for Sp1 transcriptional activation subunit 9; CRSP complex subunit 9; mediator complex subunit 7; RNA polymerase transcriptional regulation mediator subunit 7 homolog; transcriptional coactivator CRSP33 (MED7 ARC34 CRSP9) RNA polymerase 2 holoenzyme component SRB7; mediator complex subunit 21; suppressor of RNA polymerase B7 (SRB7, MED21, SURB7) thyroid hormone receptor-associated protein 6 (thyroid hormone receptor-associated protein complex 25 kD component, trap25, TRAP/mediator complex component TRAP25, MED30, THRAP6, TRAP25) ubiquitin carboxyl-terminal hydrolase 47; deubiquitinating enzyme 47; ubiquitin thiolesterase 47; ubiquitin-specific-processing protease 47 (USP47)