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cryptochrome 2 (CRY2, KIAA0658)
Function:
1) blue light-dependent regulator of the circadian feedback
2) component of the circadian core oscillator
3) inhibits CLOCK|NPAS2-BMAL1 E box-mediated transcription via stabilizing unphosphorylated form of BMAL1
4) acts, in conjunction with CRY1, in maintaining period length & circadian rhythmicity
5) no photolyase activity
6) translocates circadian clock core proteins, including PER proteins to the nucleus (putative)
7) interacts directly with PER1 & PER2 C-terminal domains
- interaction with PER2 inhibits its ubiquitination & vice versa
8) phosphorylation on Ser-266 by MAPK is important for the inhibition of CLOCK-BMAL1-mediated transcriptional activity
9) phosphorylation by CSNK1E requires interaction with PER1 or PER2
Cofactor:
1) binds 1 FAD per subunit
2) binds 1 5,10-methenyltetrahydrofolate non-covalently per subunit
Structure:
1) belongs to the DNA photolyase class-1 family
2) contains 1 DNA photolyase domain
Compartment:
- cytoplasm, nucleus
- translocated to the nucleus via interaction with other Clock proteins
Expression:
- expressed in all tissues including heart & skeletal muscle > brain, fibroblasts, placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes
General
carrier protein (transporter)
phosphoprotein
Properties
SIZE: MW = 67 kD
entity length = 593 aa
MOTIF: DNA photolyase {22-189}
binding site
SITE: 230-507
FOR-BINDING-OF: flavin adenine dinucleotide
MOTIF: Ser phosphorylation site {S266}
CLOCK-BMAL1 inhibition {390-489}
Ser phosphorylation site {S558}
Database Correlations
OMIM 603732
UniProt Q49AN0
PFAM correlations
Entrez Gene 1408
References
- UniProt :accession Q49AN0
- OMIM :accession 603732