microtubule-associated serine/threonine protein kinase 2 (MAST2, KIAA0807, MAST205)

Function: - appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins - phosphorylation of DMD or UTRN may modulate their affinities for associated proteins - functions in a multi-protein complex in spermatid maturation - regulates lipopolysaccharide-induced IL-12 synthesis in macrophages by forming a complex with TRAF6, resulting in the inhibition of TRAF6 NF-kappa-B activation (putative) - phosphorylated & ubiquitinated - N-terminal ubiquitination leads to degradation of MAST2 by proteasome-mediated proteolysis - N-terminal phosphorylation appears to be a prerequisite for ubiquitination (putative) - interacts with CDHR2 Cofactor: Mg⁺² (putative) Structure: - belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family - contains 1 AGC-kinase C-terminal domain - contains 1 PDZ domain (DHR domain) - contains 1 protein kinase domain Compartment: - cytoplasm, cytoskeleton - plasma membrane, cytoplasmic side (putative) - recruited to the sub-membranous area on interaction with CDHR2 Alternative splicing: named isoforms=2 Expression: abundant in the testis

Properties

SIZE: entity length = 1798 aa MW = 196 kD COMPARTMENT: cytoplasm STATE: active state MOTIF: Ser phosphorylation site {S74} Ser phosphorylation site {S148} Ser phosphorylation site {S151} kinase domain SITE: 512-785 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 518-526 ATP-binding site NAME: ATP-binding site SITE: 541-541 aspartate residue {D635} AGC-kinase C-terminal {786-854} Ser phosphorylation site {S900} PDZ domain NAME: PDZ domain SITE: 1104-1192 Ser phosphorylation site {S1503} Thr phosphorylation site {T1508}

Database Correlations

UniProt Q6P0Q8 PFAM correlations Entrez Gene 23139 Kegg hsa:23139 ENZYME 2.7.11.1

References

UniProt :accession Q6P0Q8