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microtubule-associated protein RP/EB family member 3; end-binding protein 3; EB3; EB1 protein family member 3 (EBF3, RP3, MAPRE3)

Function: 1) microtubule polymerization & spindle function by stabilizing microtubules & anchoring them at centrosomes 2) role in cell migration (putative) 3) interacts with DCTN1 4) binds to the C-terminal domain of APC 5) binds monomeric & polymerized tubulin 6) two functionally independent domains a) N-terminal domain forms an hydrophobic cleft involved in microtubule binding b) C-terminal involved in formation of mutually exclusive complexes with APC & DCTN1 Structure: 1) belongs to the MAPRE family 2) contains 1 CH domain (calponin-homology domain) - contains 1 EB1 C-terminal domain Compartment: cytoplasm, associated with the microtubule network Alternative splicing: named isoforms=2 Expression: expressed in brain & muscle

General

microtubule-associated protein (MAP)

Properties

SIZE: MW = 32 kD entity length = 281 aa COMPARTMENT: cytoplasm MOTIF: calponin homology domain NAME: calponin homology domain SITE: 14-116 EB1 C-terminal {194-264} binding site SITE: 217-281 FOR-BINDING-OF: dynactin-1 MOTIF: binding site SITE: 217-260 FOR-BINDING-OF: adenomatous polyposis coli protein

Database Correlations

OMIM 605788 UniProt Q9UPY8 PFAM correlations

References

UniProt :accession Q9UPY8