MAP kinase-activated protein kinase 5 (MAPK-activated protein kinase 5, MAPKAP kinase 5, p38-regulated/activated protein kinase, MAPKAPK5, PRAK)

in mice, activated by ERK/p38. regulatory light chain of myosin 2 Function: - mediates stress-induced phosphorylation of small heat shock protein 2 - phosphorylates heat shock protein 27 & regulatory light chain of myosin 2 - interacts with SQSTM1 - p38 alpha & beta-dependent phosphorylation increases its activity - in mice, activated by ERK/p38 - activated by stress-related extracellular stimuli such as H₂O₂, arsenite, anisomycin TNF alpha, & to a lesser extent PMA & the Ca⁺² ionophore A23187 - in vitro, activated by SQSTM1 - phosphorylated on Thr-182, its regulatory phosphorylation site located on the T-loop/loop 12 ATP + a protein ADP + a phosphoprotein Structure: - belongs to the protein kinase superfamily, Ser/Thr protein kinase family - contains 1 protein kinase domain Compartment: cytoplasm, nucleus. Alternative splicing: named isoforms=2 Expression: expressed ubiquitously

Properties

SIZE: entity length = 473 aa MW = 54 kD COMPARTMENT: cytoplasm cell nucleus STATE: active state MOTIF: kinase domain SITE: 22-304 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 28-36 ATP-binding site NAME: ATP-binding site SITE: 51-51 aspartate residue {D148} Thr phosphorylation site {T182} coiled coil {409-440}

Database Correlations

OMIM 606723 UniProt Q8IW41 Pfam PF00069 Entrez Gene 8550 ENZYME 2.7.11.1

References

UniProt :accession Q8IW41