MAP kinase-activated protein kinase 2 (MAPK-activated protein kinase 2, MAPKAP kinase 2, MAPKAPK-2, MK2, MAPKAPK2)

Function: - its physiological substrate seems to be HSP27/HSP25 - in vitro can phosphorylate glycogen synthase at Ser-7' & tyrosine hydroxylase (on Ser-19 & Ser-40) - phosphorylates Ser in the peptide sequence, hyd-X-R-X(2)-S, where hyd is a large hydrophobic residue - mediates both ERK & p38 MAPK/MAPK14 dependent neutrophil responses - participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils - plays a role in phagocytosis-induced respiratory burst activity - interacts with PHC2 - seems to be activated by two distinct pathways: a) stimulation of p42/p44 MAPK by growth factors b) triggered by stress & heat shock, dependent on activation of MPK2 & upstream MAPKK/MAPKKK - phosphorylated & activated by MAP kinase ATP + a protein ADP + a phosphoprotein Structure: - belongs to the protein kinase superfamily, Ser/Thr protein kinase family - contains 1 protein kinase domain Alternative splicing: named isoforms=2 Expression: expressed in all tissues examined

Properties

SIZE: entity length = 400 aa MW = 46 kD STATE: active state MOTIF: proline-rich region SITE: 10-40 MOTIF: proline residue (SEVERAL) proline-rich region SITE: 35-40 MOTIF: proline residue (SEVERAL) Tyr phosphorylation site {Y63} kinase domain SITE: 64-325 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 70-78 ATP-binding site NAME: ATP-binding site SITE: 93-93 aspartate residue {D186} Ser phosphorylation site {S328} Thr phosphorylation site {T334} Thr phosphorylation site {T338}

Database Correlations

OMIM 602006 UniProt P49137 Pfam PF00069 Entrez Gene 9261 ENZYME 2.7.11.1

References

UniProt :accession P49137