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mitogen-activated protein kinase kinase kinase 12 (MAP3K12, leucine zipper protein kinase, ZPK, dual leucine zipper bearing kinase, DLK, MAPK-upstream kinase, MUK)
Function:
- may be an activator of the JNK/SAPK pathway
- phosphorylates beta-casein, histone 1 & myelin basic protein in vitro
- interacts with MBIP
- autophosphorylated on Ser/Thr
- phosphorylated in cytosol under basal conditions & dephosphorylated when membrane-associated (putative)
- interacts with MBIP through the leucine-zipper motif
ATP + a protein ADP + a phosphoprotein
Cofactor: Mg+2
Structure:
- belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily
- contains 1 protein kinase domain
Compartment:
- cytoplasm (putative)
- peripheral membrane (putative)
Expression: highly expressed in brain & kidney
General
mitogen-activated protein kinase kinase kinase (MAP3K) or MEK kinase (MEKK)
Properties
SIZE: entity length = 859 aa
MW = 93 kD
COMPARTMENT: cytoplasm
STATE: active state
MOTIF: kinase domain
SITE: 125-366
MOTIF: ATP-binding site
NAME: ATP-binding site
SITE: 131-139
ATP-binding site
NAME: ATP-binding site
SITE: 152-152
aspartate residue {D236}
leucine zipper
SITE: 390-411
FOR-BINDING-VIA: leucine zipper
leucine zipper
SITE: 443-464
FOR-BINDING-VIA: leucine zipper
proline-rich region
SITE: 665-668
MOTIF: proline residue (SEVERAL)
glutamate-rich region {720-731}
MOTIF: glutamate residue (SEVERAL)
References
UniProt :accession Q12852
Databases & Figures
OMIM 600447
UniProt Q12852
Pfam PF00069
Entrez Gene 7786
ENZYME 2.7.11.25
MAP kinase pathways