mitogen activated protein kinase kinase 6 (dual specificity mitogen-activated protein kinase kinase 6, MAPK/ERK kinase 6, MEK6, SAPKK3, MAP2K6, MKK6, PRKMK6)

Function: - MAP2K3 & MAP2K6 activate p38MAPKs - catalyzes concomitant phosphorylation of a Thr & a Tyr in MAP kinase p38 exclusively - interacts with Yersinia yopJ - probably activated by dual phosphorylation on Ser-207 & Thr-211 - weakly autophosphorylated - acetylation of Ser-207 & Thr-211 by Yersinia yopJ prevents phosphorylation & activation, thus blocking the MAPK signaling pathway ATP + a protein = ADP + a phosphoprotein Structure: - belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase subfamily - contains 1 protein kinase domain Alternative splicing: named isoforms=2 Expression: - isoform 2 is only expressed in skeletal muscle - isoform 1 is expressed in skeletal muscle, heart > liver, pancreas - strongly activated by UV, anisomycin, & osmotic shock but not by phorbol esters, NGF or EGF

Properties

SIZE: entity length = 334 aa MW = 37 kD STATE: active state MOTIF: proteolytic site {14-15} kinase domain SITE: 53-314 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 59-67 ATP-binding site NAME: ATP-binding site SITE: 82-82 aspartate residue {D179} Ser phosphorylation site {S207} Thr phosphorylation site {T211}

References

UniProt :accession P46734

Databases & Figures

OMIM 601254 UniProt P52564 Pfam PF00069 Entrez Gene 5608 ENZYME 2.7.12.2 MAP kinase pathways