microtubule-associated protein 1S (MAP 1S, BPY2-interacting protein 1, microtubule-associated protein 8, variable charge Y chromosome 2-interacting protein 1, VCY2-interacting protein 1, protein VCY2IP-1, [contains: MAP1S heavy chain; MAP1S light chain] MAP1S, BPY2IP1, C19orf5, MAP8, VCY2IP1)

Function: - microtubule-associated protein - mediates aggregation of mitochondria resulting in cell death & genomic destruction (MAGD) - plays a role in anchoring the microtubule-organizing center to the centrosomes - binds to DNA - plays a role in apoptosis - involved in the formation of microtubule bundles - interacts with microtubules & actin - both MAP1S heavy & light chains interact with microtubules - MAP1S light chain interacts with actin - interacts with ESR1, LRPPRC, RASSF1 isoform A & isoform C, microtubules & VCY2 Structure: - heterodimer of a heavy & a light chain. - N-terminus of the heavy chain associates with the C-terminus of the light chain to form the heterodimer - its C-terminal part of the heavy chain interacts with ESR1 - belongs to the MAP1 family Compartment: - nucleus, cytoplasm, cytosol, cytoplasm, cytoskeleton - detected in filopodia-like protrusions & synapses (putative) - detected in perinuclear punctate network corresponding to mitochondrial aggregates & in the nucleus in cells exhibiting apoptosis - associated specifically with microtubules stabilized by paclitaxel & colocalizes with RASSF1 isoform A - in interphase cells, shows a diffuse cytoplasmic staining with partial localization to the microtubules - during the different stages of mitosis detected at the spindle microtubules Expression: - expressed in neurons (at protein level) - expressed in spermatocytes, spermatids & spermatozoa - expressed in the cerebral cortex - highly expressed in testis - expressed in the brain, colon, heart, kidney, liver, lung, placenta, small intestine, spleen & stomach > muscle Pathology: - depletion of MAP1S by RNAi causes mitotic abnormalities that consist of failure to form a stable metaphase plate, premature sister chromatid separation, lagging chromosomes, & multipolar spindles

General

DNA-binding protein microtubule-associated protein (MAP) phosphoprotein

Properties

SIZE: entity length = 1059 aa MW = 112 kD COMPARTMENT: cytoplasm MOTIF: microtubule-organizing center {1-797} MOTIF: Ser phosphorylation site {S472} DNA-binding motif proline-rich region SITE: 560-850 MOTIF: proline residue (SEVERAL) Thr phosphorylation site {T638} Ser phosphorylation site {S640} Ser phosphorylation site {S657} RASSF1 interaction {666-1059} MOTIF: microtubule association {714-966} Ser phosphorylation site {S729} Ser phosphorylation site {S731} Ser phosphorylation site {S759} Ser phosphorylation site {S900} actin association {960-1059} mitochondrial aggregation {967-991}

Database Correlations

OMIM 607573 UniProt Q66K74

References

UniProt :accession Q66K74