lysine-specific histone demethylase 1; amine oxidase flavin-containing domain protein 2; BRAF35-HDAC complex protein; BHC110 (AOF2, KDM1A, KIAA0601, LSD1)

Function: 1) histone demethylase 2 demethylates Lys-4 of histone H3 - demethylates both mono- & tri-methylated Lys-4 of histone H3 3) specific tag for epigenetic transcriptional activation (acts as a corepressor) 4) demethylates Lys-9 of histone H3 5) specific tag for epigenetic transcriptional repression - derepression of androgen receptor target genes 6) FAD-mediated substrate oxidation to generate imine that is subsequently hydrolyzed 7) repression of neuronal genes (putative) 8) requires the presence of RCOR1/CoREST for activity 9) component of a BHC histone deacetylase complex Cofactor: FAD Kinetic parameters: - KM=3.0 uM for H3 monomethyl-K4 - KM=4.2 uM for H3 dimethyl-K4 - KM=3.9 uM for H3 monomethyl-K4-monomethyl-K9 - KM=17.5 uM for monomethyl-K4-acetyl-K9 Structure: - SWIRN domain may act as an anchor site for a histone tail Compartment: nucleus Alternative splicing: named isoforms=2 Expression: ubiquitously expressed

General

histone demethylase oxidoreductase

Properties

SIZE: MW = 93 kD entity length = 852 aa COMPARTMENT: cell nucleus MOTIF: alanine-rich region {7-42} MOTIF: alanine residue (SEVERAL) coiled coil {110-151} proline-rich region SITE: 152-156 MOTIF: proline residue (SEVERAL) SWIRM {174-273} cofactor-binding site [281-309] COFACTOR-BOUND: flavin adenine dinucleotide active site SITE: 300-852 MOTIF: coiled coil {428-514}

Database Correlations

OMIM 609132 UniProt O60341 PFAM correlations Entrez Gene 23028 Kegg hsa:23028

References

UniProt :accession O60341