very long-chain acyl-CoA synthetase; VLACS; VLCS; fatty acid transport protein 2; FATP-2; fatty-acid-coenzyme A ligase, very long-chain 1; long-chain-fatty-acid--CoA ligase; solute carrier family 27 member 2; THCA-CoA ligase; very long-chain-fatty-acid-CoA ligase (SLC27A2, ACSVL1, FACVL1, FATP2, VLACS)

Function: - acyl-CoA synthetase involved in bile acid metabolism - activates C27 precurors of bile acids to their CoA thioesters derivatives before side chain cleavage - in vitro, activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid - does not utilize C24 bile acids as substrates - in vitro, also activates long-chain fatty acids & branched-chain fatty acids & may have additional roles in fatty acid metabolism - may be involved in translocation of long-chain fatty acids across membranes ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA ATP + a very-long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA Structure: - belongs to the ATP-dependent AMP-binding enzyme family Compartment: - endoplasmic reticulum membrane - peroxisome membrane - multi-pass membrane protein Expression: - expressed in liver, kidney, placenta & pancreas

General

fatty-acid CoA ligase phosphoprotein solute carrier family 27 (SLC27, SLC27A) transmembrane 3 protein

Properties

SIZE: entity length = 620 aa MW = 70 kD COMPARTMENT: peroxisome endoplasmic reticulum MOTIF: exoplasmic domain {1-4} transmembrane domain {5-27} cytoplasmic loop {28-106} transmembrane domain {107-127} exoplasmic loop {128-261} transmembrane domain {262-282} cytoplasmic domain {283-620} MOTIF: Thr phosphorylation site {T577}

Database Correlations

OMIM 603247 UniProt O14975 Pfam PF00501 Entrez Gene 11001 Kegg hsa:11001 ENZYME correlations

References

UniProt :accession O14975