LDL cholesterol direct (LDL direct) in serum

Used in conjunction with Synchron LX systems, Unicel DxC 600/800 systems. Indications: hypertriglyceridemia Increases: - hyperlipoproteinemia type 2B Principle: - direct quantification of LDL cholesterol in serum or plasma - homogeneous assay, using detergent that solubilizes only non-LDL lipoproteins, releasing cholesterol & cholesterol esters to react with cholesterol esterase - the result cholesterol is oxidized with cholesterol oxidase in a non-color forming reaction. - a 2nd detergent solubilizes the remaining LDL lipoprotein, releasing cholesterol & cholesterol esters to react with cholesterol esterase - the result cholesterol is oxidized with cholesterol oxidase in a reaction coupled to chromphore production - timed endpoint method monitoring absorbance at 560 nm Detergent 1 cholesterol esterase HDL-C, VLDL-C, CM-C --------------------> non color poduct cholesterol oxidase Detergent 2 cholesterol esterase LDL cholesterol --------------------> cholestenone + H₂O₂ cholesterol oxidase peroxidase H₂O₂ + DSBmT* + 4-AA* ------------> color product * DSBmT = N,N-bis (4-sulphobutyl)-m-toluidine-disodium 4-AA = 4 aminopyrine

Related

cholesterol in serum/plasma lipid panel (fasting lipid panel, FLP)

References

Veterans Administration