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lactate dehydrogenase 5 (LDH5)

Structure: - composed of 4 LDH M subunits [1] Expression: - expressed in liver & skeletal muscle [1] Laboratory: - 7% of serum LDH activity [2]

General

lactate dehydrogenase (LDH)

Properties

SIZE: MW = 134 kD COMPARTMENT: cytoplasm CELL: most cell types* WITHIN: anatomic structure* in vitro MOTIF: active site SUBUNITS: LDH M subunit (4) MOTIF: cofactor-binding site [29-57] COFACTOR-BOUND: NAD cofactor-binding site [99-99] COFACTOR-BOUND: NAD binding site SITE: 106-106 FOR-BINDING-OF: Substrate binding site SITE: 138-138 FOR-BINDING-OF: NAD or substrate binding site SITE: 169-169 FOR-BINDING-OF: Substrate histidine residue {H193} Tyr phosphorylation site {Y239} binding site SITE: 248-248 FOR-BINDING-OF: Substrate MISC-INFO: lifetime 8-12 HOURS {1/2-LIFE}

References

  1. Wikipedia, lactate dehydrogenase entry http://en.wikipedia.org/wiki/lactate_dehydrogenase
  2. Laboratory Medicine: Test Selection & Interpretation. Howanitz & Howanitz (eds), Churchill Livingstone, NY, 1991, pg 32

Components

lactate dehydrogenase A or LDH M subunit