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lactate in serum/plasma/blood

Reference values: - Male: 0.5-2.9 mmol/L - Female: 0.5-2.9 mmol/L Clinical significance: - lactate is the end product of the anaerobic metabolism of glucose - lactic acidosis results from local tissue hypoxia caused by dehydration, poor perfusion, as a result of shock (circulatory collapse) or cardiac failure - mortality risk increases with increasing plasma lactate level in patients with sepsis or suspected infection [7] Increases: - strenuous muscular exercise - systemic infections - shock - hypoxia - metabolic acidosis - diabetic ketoacidosis - nonketotic acidosis - type 1 glycogen storage disease - enzyme defects - alcoholism - neoplasia - toxicity, including salicylate poisoning Principle: The Kodak Ektachem Clinical Chemistry Slide (LAC) is a dry, multilayered, self-contained analytical element coated on a transparent polyester support. A 10 microliter drop of sample is deposited on the slide & is evenly distributed by the spreading layer. Lactate in the sample is oxidized by the lactate oxidase to pyruvate & H2O2. The H2O2 generated oxidizes the 4-aminoantipyrene, 1,7-dihydroxy- naphthalene chromogen system in a horseradish peroxidase-catalyzed reaction & results in a dye complex. The sample is incubated for 5 minutes & the intensity of the dye complex is measured spectrophotometrically. Specimen: Patient Preparation: Draw the specimen when the patient is at rest without the use of a tourniquet or within 3 minutes of applying the tourniquet, but before releasing the tourniquet. The patient should avoid any exercise of the arm or hand before or during collection of the specimen. Sample Preparation: Collect the specimen by the venipuncture technique described in patient preparation above. The combination of 2.5 mg/mL of sodium fluoride & 2.0 mg/mL of potassium oxalate is effective as an anticoagulant & antiglycolytic substance. Heparinized plasma is acceptable, but precautions must be taken to retard glycolysis by keeping the whole blood on ice & then immediately separating the plasma from the cells. Specimens must be collected in tubes at least half full. Sample requirements: Minimum- 0.5mL sodium fluoride/potassium oxalate plasma. Optimum- 1.0mL sodium fluoride/potassium oxalate plasma.

Related

lactate lactic acidosis

Specific

lactate in blood lactate in serum/plasma

General

lactate in body fluid

References

  1. Kodak Ektachem 700 Test Methodologies Manual, Kodak Clinical Products, Rochester, N.Y., 1990.
  2. Kodak Ektachem 700 Operators Manual, Kodak Clinical Products, Rochester, N.Y., 1987.
  3. Package Insert, Kodak Ektachem Special Calibrators, Kodak Clinical Products, Rochester, N.Y., 1985.
  4. Package Insert, Bio-Rad Liquichek Controls, Bio-Rad ECS Division, Anaheim, CA., 1992.
  5. Lactic Acid, Plasma Laboratory Test Directory ARUP: 20045
  6. Panel of 10 tests Laboratory Test Directory ARUP: 99289
  7. Puskarich MA et al. Prognostic value of incremental lactate elevations in emergency department patients with suspected infection. Acad Emerg Med 2012 Aug; 19:983 PMID: 22905962

Component-of

coma panel