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lactate dehydrogenase (LDH) in body fluid

Clinical significance: Lactate dehydrogenase is an enzyme present in the cytosol of the human cells; it catalyzes the reversible reduction of pyruvate to lactate using NADH. Principle: The Kodak Ektachem Clinical Chemistry Slide (LDH) is a dry, multilayered, self-contained analytical element coated on a clear polyester support. The assay for lactate dehydrogenase (LDH) uses pyruvate & NADH as substrates to generate lactate & NAD. An 11 microliter drop of sample is deposited on the slide & evenly distributed by the spreading layer. Lactate dehydrogenase catalyzes the conversion of pyruvate & NADH to lactate & NAD. The oxidation of NADH, which is monitored by reflectance spectrophotometry, is used to measure lactate dehydrogenase activity. Preparation: No special patient preparation is necessary. Specimen: - see lactate dehydrogenase in serum

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lactate dehydrogenase (LDH)

Specific

lactate dehydrogenase (LDH) in amniotic fluid lactate dehydrogenase (LDH) in CSF lactate dehydrogenase (LDH) in dialysis fluid lactate dehydrogenase (LDH) in gastric fluid lactate dehydrogenase (LDH) in pericardial fluid lactate dehydrogenase (LDH) in peritoneal fluid lactate dehydrogenase (LDH) in pleural fluid lactate dehydrogenase (LDH) in semen lactate dehydrogenase (LDH) in serum lactate dehydrogenase (LDH) in synovial fluid lactate dehydrogenase (LDH) in urine

General

lactate dehydrogenase (LDH) measurement

References

  1. Kodak Ektachem 700 Test Methodologies Manual, Kodak Clinical Products, Rochester, N.Y., 1990.
  2. Kodak Ektachem 700 Operators Manual, Kodak Clinical Products, Rochester, N.Y., 1987.
  3. Package Insert, Kodak Ektachem Special Calibrators, Kodak Clinical Products, Rochester, N.Y., 1985.
  4. Package Insert, Bio-Rad Liquichek Controls, Bio-Rad ECS Division, Anaheim, CA., 1992.
  5. Clinical Diagnosis & Management by Laboratory Methods, 19th edition, J.B. Henry (ed), W.B. Saunders Co., Philadelphia, PA. 1996, pg 11.
  6. Lactate Dehydrogenase Total, Body Fluid Laboratory Test Directory ARUP: 20505