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myosin light chain kinase smooth musle (MLCK, MYLK, telokin, kinase-related protein, KRP)

Function: 1) smooth muscle contraction via phosphorylation of Ser in the N-terminus of myosin light chains 2) growth of astrocytic processes 3) transmitter release at synapses of sympathetic ganglion neurons 4) fibroblast apoptosis 5) precursor to several splice products: telokin, non-muscle MLCK isozyme & smooth muscle MLCK isozyme 6) responsible for smooth muscle contraction via phosphorylation of specific Ser of myosin light chains

General

myosin light chain kinase (MLCK, MYLK)

Properties

SIZE: gel filtration = 124 kD COMPARTMENT: cytoplasm STATE: active state MOTIF: S/T-phosphate calmodulin binding site EFFECTOR-BOUND: calmodulin consensus repeat {868-1063} (11) kinase domain SITE: 1464-1719 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 1470-1478 ATP-binding site NAME: ATP-binding site SITE: 1493-1493 aspartate residue {D1585} calmodulin binding site SITE: 1711-1774 FOR-BINDING-OF: calmodulin immunoglobulin superfamily domain {1824-1891} glutamate residue {1906-1914} (9) MISC-INFO: DISTRIBUTION :REGIONAL BROAD :SUBCELLULAR CYTOSOLIC SUBSTRATE-SPECIFICITY :RANGE NARROW calmodulin binding site :SITE C-TERMINAL RECOGNITION-MOTIF :SEQUENCE XKKRXXRXX[S*]X :REF " - Kemp BE, Pearson RB. Protein kinase recognition sequence motifs. Trends Biochem Sci. 1990 Sep;15(9):342-6. Review. PMID: 2238044"

Database Correlations

OMIM 600922 UniProt Q15746 ENZYME 2.7.11.18

References

UniProt :accession Q15746