kinetochore protein Hec1 (HsHec1, kinetochore-associated protein 2, highly expressed in cancer protein, retinoblastoma-associated protein HEC, KNTC2, HEC, HEC1)

Function: 1) acts as a component of the essential kinetochore-associated NDC80 complex 2) kinetochore integrity & organization of stable microtubule binding sites in outer plate of kinetochore 3) interacts with NEK2 & ZWINT specifically during mitosis 4) interacts with CENPH & MIS12 5) interacts with AURKB, PSMC2, PSMC5, SMC1L1 (putative) 6) interacts with RB1 during G2 phase & mitosis (putative) 7) phosphorylation begins in S phase of cell cycle & peaks in mitosis 8) phosphorylated by NEK2 9) may also be phosphorylated by AURKA & AURKB Compartment: - nucleus, kinetochore - localizes to kinetochores from late prophase to anaphase - localizes specifically to the outer plate of kinetochore Expression: - peaks in mitosis

General

kinetochore protein phosphoprotein

Properties

SIZE: MW = 74 kD entity length = 642 aa COMPARTMENT: cell nucleus MOTIF: binding site SITE: 1-445 FOR-BINDING-OF: Kinetochore protein Nuf2 MOTIF: nuclear localization {1-250} Ser phosphorylation site {S55} Ser phosphorylation site {S62} Ser phosphorylation site {S76} Ser phosphorylation site {S77} RB1 interaction {128-251} Ser phosphorylation site {S165} interaction with NEK2 & ZWINT {251-618} MOTIF: binding site SITE: 251-431 FOR-BINDING-OF: structural maintenance of chromosome 1-like 1 protein coiled coil {261-403} binding site SITE: 361-547 FOR-BINDING-OF: MSS1 protein structural maintenance of chromosome 1-like 1 protein binding site SITE: 446-642 FOR-BINDING-OF: testis-specific Y-encoded-like protein 2 MOTIF: coiled coil {458-642}

Database Correlations

OMIM 607272 UniProt O14777 Pfam PF03801

References

UniProt :accession O14777