antigen KI-67 (MKI67)

Function: - thought to be required for maintaining cell proliferation - interacts with KIF15 - binds through the FHA domain to MKI67IP Structure: - contains 1 FHA domain - contains many Ser/Thr phosphorylation sites Compartment: - nucleus - predominantly localized in G1 phase in perinucleolar region - in later phases, detected throughout the nuclear interior, predominantly localized in the nuclear matrix - in mitosis, present on all chromosomes Alternative splicing: named isoforms=2; long, short Expression: - expression occurs preferentially during late G1 phase, S phase, G2 phase & M phases of the cell cycle - not detected G0 phase Pathology: - MIB-1 is the name of an Ig-G1 antibody against Ki67 which works well in formalin fixed tissues, often used to determine proliferation index of tumors - may help distinguish benign from malignant adrenal cortical tumors

General

nuclear protein phosphoprotein

Properties

SIZE: entity length = 3256 aa MW = 359 kD COMPARTMENT: cell nucleus MOTIF: forkhead-associated (FHA) domain {27-76} Ser phosphorylation site {S108} Thr phosphorylation site {T109} Ser phosphorylation site {S125} Ser phosphorylation site {S128} Ser phosphorylation site {S131} Ser phosphorylation site {S264} Ser phosphorylation site {S308} Thr phosphorylation site {T328} Thr phosphorylation site {T347} Ser phosphorylation site {S352} Ser phosphorylation site {S357} Ser phosphorylation site {S374} Thr phosphorylation site {T401} Ser phosphorylation site {S411} Ser phosphorylation site {S538} Thr phosphorylation site {T543} Ser phosphorylation site {S579} Ser phosphorylation site {S584} Ser phosphorylation site {S630} Ser phosphorylation site {S648} Ser phosphorylation site {S713} Thr phosphorylation site {T761} Ser phosphorylation site {S827} Ser phosphorylation site {S859} consensus repeat {1000-1112} MOTIF: Thr phosphorylation site {T1017} Ser phosphorylation site {S1098} Thr phosphorylation site {T1111} consensus repeat {1122-1234} MOTIF: Ser phosphorylation site {S1131} Thr phosphorylation site {T1139} Thr phosphorylation site {T1176} Thr phosphorylation site {T1193} Ser phosphorylation site {S1207} Thr phosphorylation site {T1233} Thr phosphorylation site {T1237} consensus repeat {1244-1356} MOTIF: Ser phosphorylation site {S1253} Ser phosphorylation site {S1256} Thr phosphorylation site {T1261} Thr phosphorylation site {T1298} Ser phosphorylation site {S1302} Thr phosphorylation site {T1315} Thr phosphorylation site {T1327} Ser phosphorylation site {S1329} Thr phosphorylation site {T1335} Thr phosphorylation site {T1355} consensus repeat {1366-1477} MOTIF: Ser phosphorylation site {S1375} Ser phosphorylation site {S1376} Thr phosphorylation site {T1383} consensus repeat {1487-1598} MOTIF: Thr phosphorylation site {T1503} Ser phosphorylation site {S1506} Thr phosphorylation site {T1540} Ser phosphorylation site {S1546} Tyr phosphorylation site {Y1552} Thr phosphorylation site {T1557} Thr phosphorylation site {T1565} Thr phosphorylation site {T1569} Ser phosphorylation site {S1571} consensus repeat {1608-1720} MOTIF: Ser phosphorylation site {S1618} Ser phosphorylation site {S1628} Ser phosphorylation site {S1629} Ser phosphorylation site {S1636} Ser phosphorylation site {S1679} Ser phosphorylation site {S1689} Thr phosphorylation site {T1719} Ser phosphorylation site {S1721} Ser phosphorylation site {S1726} consensus repeat {1730-1842} MOTIF: Ser phosphorylation site {S1740} Thr phosphorylation site {T1747} Thr phosphorylation site {T1764} Thr phosphorylation site {T1784} Thr phosphorylation site {T1801} Ser phosphorylation site {S1815} Thr phosphorylation site {T1841} consensus repeat {1851-1964} MOTIF: Ser phosphorylation site {S1861} Ser phosphorylation site {S1864} Thr phosphorylation site {T1869} Thr phosphorylation site {T1897} Thr phosphorylation site {T1923} Ser phosphorylation site {S1937} consensus repeat {1974-2086} MOTIF: Ser phosphorylation site {S1983} Ser phosphorylation site {S2002} Thr phosphorylation site {T2065} Ser phosphorylation site {S2072} Thr phosphorylation site {T2085} consensus repeat {2096-2204} MOTIF: Ser phosphorylation site {S2105} Thr phosphorylation site {T2113} Ser phosphorylation site {S2135} Thr phosphorylation site {T2203} Thr phosphorylation site {T2211} consensus repeat {2214-2326} MOTIF: Ser phosphorylation site {S2223} Thr phosphorylation site {T2231} Ser phosphorylation site {S2239} Thr phosphorylation site {T2285} Ser phosphorylation site {S2299} Thr phosphorylation site {T2305} Thr phosphorylation site {T2325} Thr phosphorylation site {T2328} Thr phosphorylation site {T2332} consensus repeat {2335-2447} MOTIF: Ser phosphorylation site {S2344} Thr phosphorylation site {T2352} Ser phosphorylation site {S2355} Thr phosphorylation site {T2389} Ser phosphorylation site {S2395} Thr phosphorylation site {T2406} Ser phosphorylation site {S2420} Thr phosphorylation site {T2426} Thr phosphorylation site {T2446} consensus repeat {2457-2569} MOTIF: Ser phosphorylation site {S2466} Ser phosphorylation site {S2471} Ser phosphorylation site {S2505} Thr phosphorylation site {T2508} Thr phosphorylation site {T2509} Ser phosphorylation site {S2528} consensus repeat {2579-2689} MOTIF: Ser phosphorylation site {S2588} Ser phosphorylation site {S2638} consensus repeat {2699-2808} MOTIF: Ser phosphorylation site {S2708} consensus repeat {2818-2928} MOTIF: Ser phosphorylation site {S2827} Ser phosphorylation site {S2828} ATP-binding site NAME: ATP-binding site SITE: 3034-3041 MOTIF: Ser phosphorylation site {S3041} Ser phosphorylation site {S3042} Ser phosphorylation site {S3082} Ser phosphorylation site {S3207} Thr phosphorylation site {T3213} Ser phosphorylation site {S3216} Thr phosphorylation site {T3223}

Database Correlations

OMIM 176741 UniProt P46013 PFAM correlations Entrez Gene 4288 Kegg hsa:4288