IXL mediator complex (mediator of RNA polymerase 2 transcription)

Function: 1) can either repress or activate transcription 2) essential for basal & regulated expression of nearly all RNA polymerase 2-dependent genes 3) may act as a bridge, conveying regulatory information from enhancers & other control elements to the promoter Structure: - consists of: - NUT2, MED25, IXL/MED29, LCMR1/MED19, MED28, CRSP7/MED26, CDC2L6 & THRAP2/MED13L

Properties

SUBUNITS: NUT2 mediator of RNA polymerase 2 transcription subunit 25 COMPARTMENT: cell nucleus MOTIF: mediator complex interaction {1-226} proline-rich region SITE: 200-381 MOTIF: proline residue (SEVERAL) VP16 interaction {389-543} CREBBP interaction {395-545} RARA interaction {564-653} proline-rich region SITE: 565-735 MOTIF: proline residue (SEVERAL) RARA interaction {640-707} LXXLL {646-650} mediator of RNA polymerase 2 transcription subunit 29 COMPARTMENT: cell nucleus MOTIF: glutamine-rich region {29-84} MOTIF: glutamine residue (SEVERAL) LCMR mediator of RNA polymerase 2 transcription subunit 28 COMPARTMENT: cytoplasm cell nucleus MOTIF: coiled coil {109-145} mediator of RNA polymerase II transcription subunit 26 COMPARTMENT: cell nucleus MOTIF: TFIIS N-terminal {10-87} proline-rich region SITE: 228-325 MOTIF: proline residue (SEVERAL) Ser phosphorylation site {S314} Ser phosphorylation site {S337} Ser phosphorylation site {S361} Ser phosphorylation site {S447} cyclin-dependent kinase 19 STATE: active state MOTIF: kinase domain SITE: 21-335 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 27-35 ATP-binding site NAME: ATP-binding site SITE: 52-52 aspartate residue {D151} glutamine-rich region {372-404} MOTIF: glutamine residue (SEVERAL) Ser phosphorylation site {S445} Ser phosphorylation site {S449} serine-rich region {461-491} MOTIF: serine residue (SEVERAL) THRAP

References

UniProt :accession Q9Y5B9

Components

cyclin-dependent kinase-19 (CDK19); CDC2-related protein kinase 6; cell division cycle 2-like protein kinase 6; cell division protein kinase 19; cyclin-dependent kinase 11; death-preventing kinase (CDC2L6, CDK11, KIAA1028) mediator of RNA polymerase 2 transcription subunit 25 (mediator complex subunit 25, activator-recruited cofactor 92 kD component, ARC92, activator interaction domain 1 protein, p78, MED25, ACID,1 ARC92, PTOV2, TCBAP0758) mediator of RNA polymerase 2 transcription subunit 28; endothelial-derived protein 1; mediator complex subunit 28; Merlin & Grb2-interacting cytoskeletal protein; Magicin; tumor angiogenesis marker EG-1 (MED28, EG1, FKSG20) mediator of RNA polymerase 2 transcription subunit 29 (mediator complex subunit 29, intersex-like protein, IXL, MED29) mediator of RNA polymerase II transcription subunit 26; activator-recruited cofactor 70 kD component; ARC70; cofactor required for Sp1 transcriptional activation subunit 7; CRSP complex subunit 7; mediator complex subunit 26; transcriptional coactivator CRSP70 (MED26, ARC70, CRSP7)