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indirect antiglobulin test (IAT, indirect Coomb's test)
Clinical significance: ,Agglutination of erythrocytes by specific antihuman antibodies after an in vitro incubation period (when the direct antiglobulin test is negative) indicating that plasma/serum contains anti- erythrocytic antibodies. Depending upon the application, patient, donor or reagent erythrocytes may be used.
Indirect antiglobilin test is an in vitro test for:
1) detection & identification of erythrocyte antibodies in sera (type & screen)
2) typing of erythrocyte antigens
3) cross-matching
A positive indirect antiglobilin test from a patient specimen does NOT make a diagnosis of hemolysis! A positive test may occur as a result of recent transfusion of red blood cells.
Related
gel microtube test
General
anti-human globulin test (Coomb's test)
indirect antibody test
References
- Clinical Diagnosis & Management by Laboratory Methods,
19th edition, J.B. Henry (ed), W.B. Saunders Co., Philadelphia,
PA. 1996, pg 777-780
- Medical Knowledge Self Assessment Program (MKSAP) 11, American
College of Physicians, Philadelphia 1998