interleukin enhancer-binding factor 3; nuclear factor of activated T-cells 90 kD; NF-AT-90; double-stranded RNA-binding protein 76; DRBP76; translational control protein 80; TCP80; nuclear factor associated with dsRNA; NFAR; M-phase phosphoprotein 4 (ILF3, MPP4, DRBF, MPHOSPH4, NF90)

Function: - may facilitate double-stranded RNA-regulated gene expression at the level of post-transcription - can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) & other mRNAs - functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes - can regulate protein arginine N-methyltransferase 1 activity - may regulate transcription of the IL2 gene during T-cell activation - can promote formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA - interacts with FUS & SMN proteins & with PRMT1 - forms a complex with ILF2 - can also bind to PRKDC/XRCC7: this may stabilize the interaction of PRKDC/XRCC7 & the heterodimeric complex of G22P1/KU70 & XRCC5/KU80 - forms a heteromeric complex with ZNF346 & ILF3 - found in a nuclear export complex with XPO5, ILF3, Ran & double-stranded RNA or double-stranded minihelix VA1 RNA - found in a nuclear export complex with XPO5, RAN, ILF3, ZNF346 & double-stranded RNA - interacts with XPO5 & ZNF346 - phosphorylated by RNA-dependent protein kinase (EIF2AK2) - methylated by protein arginine N-methyltransferase 1 - Arg-609 is dimethylated, probably to asymmetric dimethylarginine Structure: - contains 2 DRBM (double-stranded RNA-binding) domains - contains 1 DZF domain Compartment: nucleus, nucleolus Alternative splicing: named isoforms=6 Expression: ubiquitous

General

interleukin enhancer binding factor M-phase phosphoprotein

Properties

SIZE: entity length = 894 aa MW = 95 kD COMPARTMENT: cell nucleus MOTIF: Ser phosphorylation site {S62} DZF {94-342} MOTIF: Ser phosphorylation site {S190} nuclear translocation signal {371-389} MOTIF: Ser phosphorylation site {S382} Ser phosphorylation site {S384} lysine-rich region {385-389} MOTIF: lysine residue (SEVERAL) DRBM 1 {398-467} Ser phosphorylation site {S482} Thr phosphorylation site {T486} DRBM 2 {524-590} Thr phosphorylation site {T592} PRMT1 interaction {609-894} MOTIF: proline-rich region SITE: 634-637 MOTIF: proline residue (SEVERAL) glycine-rich region {640-659} glycine-rich region {701-709} glycine-rich region {794-798} Ser phosphorylation site {S812}

Database Correlations

OMIM 603182 UniProt Q12906 PFAM correlations Entrez Gene 3609 Kegg hsa:3609

References

UniProt :accession Q12906