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interleukin-12 subunit beta; IL-12B; cytotoxic lymphocyte maturation factor 40 kD subunit; CLMF p40; IL-12 subunit p40; NK cell stimulatory factor chain 2; NKSF2 (IL12B NKSF2)

Function: - cytokine - can act as a growth factor for activated T-cells & NK cells, enhance lytic activity of NK/lymphokine-activated killer cells, & stimulate the production of IFN-gamma by resting lymphocytes - associates with IL23A to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate immunity & adaptive immunity - IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues - IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 & IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T- cells & promotes production of proinflammatory cytokines - IL-23 induces autoimmune inflammation & thus may be responsible for autoimmune inflammatory diseases & may be important for tumorigenesis Structure: - C-mannosylated in the recombinant protein - not yet known for sure if the wild-type protein is also modified - heterodimer with IL12A, disulfide-linked as interleukin-12 - heterodimer with IL23A, disulfide-linked as interleukin-23 - also secreted as a monomer - belongs to the type I cytokine receptor family, type 3 subfamily - contains 1 fibronectin F3 module - contains 1 Ig-like C2-type domain Pathology: - defects in IL12B are a cause of mendelian susceptibility to mycobacterial disease (MSMD) - polymorphisms in IL12B are a cause of susceptibility to psoriasis type 11

General

protein subunit

Properties

SIZE: entity length = 328 aa MW = 37 kD MOTIF: signal sequence {1-22} immunoglobulin superfamily domain {23-106} MOTIF: cysteine residue {C50} MODIFICATION: cysteine residue {C90} cysteine residue {C90} MODIFICATION: cysteine residue {C50} cysteine residue {C131} MODIFICATION: cysteine residue {C142} N-glycosylation site {N135} cysteine residue {C142} MODIFICATION: cysteine residue {C131} cysteine residue {C170} MODIFICATION: cysteine residue {C193} cysteine residue {C193} MODIFICATION: cysteine residue {C170} cysteine residue {C199} MODIFICATION: cysteine residue {C96 IN IL12A} N-glycosylation site {N222} fibronectin type III domain or F3 module {235-327} MOTIF: cysteine residue {C300} MODIFICATION: cysteine residue {C327} cysteine residue {C327} MODIFICATION: cysteine residue {C300}

Database Correlations

OMIM correlations UniProt P29460 Pfam PF10420 Entrez Gene 3593 Kegg hsa:3593

References

  1. UniProt :accession P29460
  2. IL12Bbase; Note: IL12B mutation db http://bioinf.uta.fi/IL12Bbase/
  3. GeneReviews http://www.ncbi.nlm.nih.gov/sites/genetests/lab/gene/IL12B
  4. SeattleSNPs http://pga.gs.washington.edu/data/il12b/

Component-of

interleukin-23 (IL23)