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heparan sulfate glucosamine 3-O-sulfotransferase 5; heparan sulfate D-glucosaminyl 3-O-sulfotransferase 5; 3-OST-5; heparan sulfate 3-O-sulfotransferase 5; h3-OST-5 (HS3ST5, 3OST5, HS3OST5)
Function:
- rate limiting enzyme for synthesis of Hsact
- like HS3ST1, modifies heparan sulfate to complete the structure of the antithrombin pentasaccharide binding site, thus conferring anticoagulant activity
- also generates GlcUA-GlcNS or IdoUA-GlcNS & IdoUA2S-GlcNH2
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
Structure: belongs to the sulfotransferase 1 family
Compartment:
- Golgi apparatus membrane
- single-pass type 2 membrane protein (probable)
Expression:
- highly expressed in skeletal muscle & fetal brain
- also found in adult brain, spinal cord, cerebellum & colon
Pathology:
- substrate-specific O-sulfation generates an enzyme-modified heparan sulfate which acts as a binding receptor to Herpes simplex virus-1 (HSV-1) & permits its entry
Related
glucosamine (Cosamin, Osteo-Bi-Flex)
heparan sulfate
General
glycoprotein
membrane protein
sulfotransferase
Properties
SIZE: entity length = 346 aa
MW = 40 kD
COMPARTMENT: golgi
MOTIF: transmembrane domain {13-32}
N-glycosylation site {N287}
binding site
SITE: 293-293
FOR-BINDING-OF: 3'-phosphoadenosine-5'-phosphosulfate
cysteine residue {C294}
MODIFICATION: cysteine residue {C304}
cysteine residue {C304}
MODIFICATION: cysteine residue {C294}
Database Correlations
OMIM 609407
UniProt Q8IZT8
Pfam PF00685
Entrez Gene 222537
Kegg hsa:222537
ENZYME 2.8.2.23
References
- UniProt :accession Q8IZT8
- GGDB; Note: glycogene database
http://riodb.ibase.aist.go.jp/rcmg/ggdb/