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heterogeneous nuclear ribonucleoprotein K; hnRNP K; transformation up-regulated nuclear protein; TUNP (HNRNPK, HNRPK)
Function:
- major pre-mRNA-binding protein
- binds tenaciously to poly(C) sequences
- likely to play a role in nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences
- can also bind poly(C) single-stranded DNA
- Arg-296 & Arg-299 are dimethylated, probably to asymmetric dimethylarginine
- interacts with RBM42 & ZIK1 (putative)
- identified in the spliceosome C complex
- interacts with HCV core protein
- interacts with ASFV p30 protein
Structure:
- contains 3 KH domains
- mass=50976.25 by mass spec [2]
Compartment:
- cytoplasm. nucleus, nucleoplasm
Alternative splicing: named isoforms=3
General
DNA-binding protein
heterogeneous nuclear ribonucleoprotein (hnRNP)
phosphoprotein
RNA-binding protein
Properties
SIZE: entity length = 463 aa
MW = 51 kD
COMPARTMENT: cytoplasm
cell nucleus
MOTIF: acetylation site
SITE: N-TERMINUS
EFFECTOR-BOUND: acetyl
Thr phosphorylation site {T3}
ASFV p30 interaction {35-197}
MOTIF: Ser phosphorylation site {S36}
KH domain
SITE: 42-104
FOR-BINDING-OF: ribonucleic acid
consensus repeat {54-76}
consensus repeat {59-62}
SEQUENCE-IS: GXGG
Ser phosphorylation site {S116}
KH domain
SITE: 144-209
FOR-BINDING-OF: ribonucleic acid
ZIK1 interaction {209-337}
MOTIF: Ser phosphorylation site {S214}
Ser phosphorylation site {S216}
binding site
SITE: 236-273
FOR-BINDING-OF: ribonucleic acid
consensus repeat {245-250}
consensus repeat {257-260}
SEQUENCE-IS: GXGG
consensus repeat {267-270}
SEQUENCE-IS: GXGG
Ser phosphorylation site {S284}
proline-rich region
SITE: 289-294
MOTIF: proline residue (SEVERAL)
consensus repeat {295-298}
SEQUENCE-IS: GXGG
proline-rich region
SITE: 310-315
MOTIF: proline residue (SEVERAL)
consensus repeat {324-329}
Ser phosphorylation site {S379}
KH domain
SITE: 387-451
FOR-BINDING-OF: ribonucleic acid
MOTIF: consensus repeat {399-421}
consensus repeat {404-407}
SEQUENCE-IS: GXGG
Database Correlations
OMIM 600712
UniProt P61978
PFAM correlations
Entrez Gene 3190
Kegg hsa:3190
References
- UniProt :accession P61978
- Harris RA et al,
Cluster analysis of an extensive human breast cancer cell line
protein expression map database.
Proteomics. 2002 Feb;2(2):212-23.
PMID: 11840567
Component-of
spliceosome C complex