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HIV1 RNA viral load

Indications: - assessment of HIV1 infection - AIDS-defining condition - Kaposi sarcoma - CNS lymphoma - pneumocystis pneumonia - progressive multifocal leukoencephalopathy - screening for HIV1* * follow-up of negative HIV1 serology with HIV1 p24 antigen & HIV1 RNA improves detaction rates in high-risk populations [2] Procedure: 1) branched DNA (bDNA) assay - generally give 1/2 the HIV RNA levels of RT-PCR 2) Reverse-transcriptase polymerase chain reaction (RT-PCR) a) Roche Amplicor ultrasensitive test b) Roche TaqMan HIV-1 3) lower limit of detection 20-50 copies/mL Specimen: 1) whole blood (ACD or EDTA) 2) store at 4 degrees C Notes: - PCR primers are directed towards env, gag & pol regions of the HIV genome - it is possible to monitor infection during the latent period

Related

human immunodeficiency virus-1 (HIV-1)

Specific

APTIMA HIV1 RNA

General

HIV1 nucleic acid

References

  1. Lima V et al. Increased reporting of detectable plasma HIV-1 RNA levels at the critical threshold of 50 copies per milliliter with the Taqman assay in comparison to the Amplicor assay. J Acquir Immune Defic Syndr 2009 Feb 25; [e-pub ahead of print]. PMID: 19247185
  2. Mini Panel of 2 tests: HIV-1 RNA Quant bDNA, Log . HIV-1 RNA Quant bDNA, Copy Laboratory Test Directory ARUP: 20466 - Panel of 3 tests Laboratory Test Directory ARUP: 55598 - HIV 1 DNA PCR, Qualitative Laboratory Test Directory ARUP: 93061 - Mini Panel of 2 tests: HIV-1 RNA Quant bDNA, Log . HIV-1 RNA Quant bDNA, Copy Laboratory Test Directory ARUP: 2002688 - Panel of 3 tests Laboratory Test Directory ARUP: 2002689
  3. Peters PJ, Westheimer E, Cohen S et al. Screening yield of HIV antigen/antibody combination and pooled HIV RNA testing for acute HIV infection in a high- prevalence population. JAMA 2016 Feb 16; 315:682 PMID: 26881371

Component-of

HIV panel