Contents

Search

HIV-1 coreceptor tropism (Trofile)

Indications: - HIV1 infection Clinical significance: 1) determines eligibility for maraviroc (Selzentry) therapy by determining variations in the HIV-1 envelope V3 loop by PCR [1] 2) HIV1 tropism testing determines ability of HIV-1 isolate to infect & replicate in monocytes [3] 3) indirectly measures ability of HIV1 to gain access to monocytes via chemokine receptor CCR5 Laboratory: - LOINC: 50790-5, 53923-9, 57182-8

Specific

HIV-1 proviral DNA tropism HIV-1 RNA tropism

General

HIV1 nucleic acid

References

  1. HIV-1 Coreceptor tropism Quest Diagnostics http://www.nicholsinstitute.com/Documents/Library/TS2457-HS.pdf
  2. Prosperi MCF et al Comparative determination of HIV-1 coreceptor tropism by Enhanced Sensitivity Trofile, gp120 V3-loop RNA and DNA genotyping Retrovirology 2010, 7:56 doi:10.1186/1742-4690-7-56 http://www.retrovirology.com/content/7/1/56
  3. O'Brien WA et al HIV-1 tropism for mononuclear phagocytes can be determined by regions of gp120 outside the CD4-binding domain Nature 1990, 348:69-73 PMID: 2172833