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HIV1 Ab in serum/plasma/blood; (HIV1 EIA/ELISA)
Indications:
- screening for HIV1
Method:
- enzyme immunoassay (EIA)
EIA is the most common method for screening individuals & blood products for antibodies to HIV. Antibodies usually appear by week 4-12 of infection, but seronegativity may be prolonged.
Additionally, antibodies may diminish as the disease process progresses. Thus a negative EIA does not rule out disease.
EIA's for HIV are 99+% sensitive for antibody to the virus, but false positives may occur with specimens that are RPR positive or from patients with hemophilia or on dialysis. EIA is generally done in duplicate & duplicate negatives are considered negative. Positive EIA's are confirmed by western blot.
Specimen:
- serum, plasma, saliva, CSF, body fluid
Related
HIV laboratory testing
human immunodeficiency virus-1 (HIV-1)
human immunodeficiency virus-1 (HIV1) western blot
General
HIV1 serology; HIV1 antibody (HIV1 EIA/ELISA, immunoblot)
References
- Clinical Guide to Laboratory Tests, 3rd edition, NW Tietz
ed, WB Saunders, Philadelphia, 1995
- HIV-1 Antibody Confirm, Western Blot
Laboratory Test Directory ARUP: 20284
- HIV-1 Antibody
Laboratory Test Directory ARUP: 2005375