Search
granzyme-A; granzyme-1; fragmentin-1; Hanukkah factor; cytotoxic T lymphocyte-associated serine esterase-3; CTLA-3; cytotoxic T-lymphocyte proteinase 1; CTL tryptase (GZMA, CTLA3, HFSP)
Function:
- necessary for target cell lysis in cell-mediated immune responses
- cleaves after Lys or Arg
- may be involved in apoptosis
- trypsin-like specificity
- hydrolysis of proteins, including fibronectin, type IV collagen & nucleolin
- preferential cleavage: -Arg-|-Xaa-, -Lys-|-Xaa- >> -Phe-|-Xaa- in small molecule substrates
Structure:
- homodimer, disulfide-linked
- belongs to the peptidase S1 family, Granzyme subfamily
- contains 1 peptidase S1 domain
Compartment: secreted, cytoplasmic granule
General
granzyme, cytotoxic T-cell protease; CTL tryptase; fragmentin
Properties
SIZE: entity length = 262 aa
MW = 29 kD
COMPARTMENT: cytoplasm
MOTIF: signal sequence {1-26}
S1 domain {29-259}
MOTIF: cysteine residue {C54}
MODIFICATION: cysteine residue {C70}
histidine residue {H69}
cysteine residue {C70}
MODIFICATION: cysteine residue {C54}
aspartate residue {D114}
cysteine residue {C148}
MODIFICATION: cysteine residue {C218}
N-glycosylation site {N170}
cysteine residue {C179}
MODIFICATION: cysteine residue {C197}
cysteine residue {C197}
MODIFICATION: cysteine residue {C179}
cysteine residue {C208}
MODIFICATION: cysteine residue {C234}
serine residue {S212}
cysteine residue {C218}
MODIFICATION: cysteine residue {C148}
cysteine residue {C234}
MODIFICATION: cysteine residue {C208}
Database Correlations
OMIM 140050
UniProt P12544
Pfam PF00089
Entrez Gene 3001
KEGG correlations
ENZYME 3.4.21.78
References
- Smyth MJ, Trapani JA.
Granzymes: exogenous proteinases that induce target cell apoptosis.
Immunol Today. 1995 Apr;16(4):202-6. Review.
PMID: 7734049
- UniProt :accession P12544