Search
general transcription factor IIF subunit 1; general transcription factor IIF 74 kD subunit; transcription initiation factor IIF subunit alpha; TFIIF-alpha; transcription initiation factor RAP74 (GTF2F1, RAP74)
Function:
- large subunit of TFIIF
- serum response factor (SRF) binding to RAP74 may be important for SRF-activated transcription
- interacts with RAP30 & CTDP1
- interacts with TAF6/TAFII80
- phosphorylated on Ser & other residues by TAF1 & casein kinase 2-like kinases
- may have serine/threonine kinase activity & autophosphorylate on Ser-385 & Thr-389 [2]
Structure: belongs to the TFIIF alpha subunit family
Compartment: nucleus
General
phosphoprotein
transcription factor IIF subunit
Properties
SIZE: entity length = 517 aa
MW = 58 kD
COMPARTMENT: cell nucleus
MOTIF: acetylation site
SITE: N-TERMINUS
EFFECTOR-BOUND: acetyl
Ser phosphorylation site {S217}
Ser phosphorylation site {S218}
Ser phosphorylation site {S221}
Ser phosphorylation site {S224}
glutamate-rich region {283-350}
MOTIF: glutamate residue (SEVERAL)
Ser phosphorylation site {S305}
Ser phosphorylation site {S307}
Ser phosphorylation site {S308}
Ser phosphorylation site {S311}
Thr phosphorylation site {T331}
Ser phosphorylation site {S342}
Ser phosphorylation site {S345}
Ser phosphorylation site {S347}
Ser phosphorylation site {S348}
Ser phosphorylation site {S351}
Ser phosphorylation site {S355}
Ser phosphorylation site {S377}
Ser phosphorylation site {S380}
Ser phosphorylation site {S381}
Ser phosphorylation site {S385}
Thr phosphorylation site {T389}
Ser phosphorylation site {S391}
Ser phosphorylation site {S431}
Ser phosphorylation site {S433}
Ser phosphorylation site {S436}
Ser phosphorylation site {S442}
Thr phosphorylation site {T445}
Database Correlations
OMIM 189968
UniProt P35269
Pfam PF05793
Entrez Gene 2962
Kegg hsa:2962
References
- Joliot V, Demma M, Prywes R.
Interaction with RAP74 subunit of TFIIF is required for
transcriptional activation by serum response factor.
Nature. 1995 Feb 16;373(6515):632-5.
PMID: 7854423
- Rossignol M, Keriel A, Staub A, Egly JM.
Kinase activity and phosphorylation of the largest subunit of
TFIIF transcription factor.
J Biol Chem. 1999 Aug 6;274(32):22387-92.
PMID: 10428810
- UniProt :accession P35269
Component-of
transcription factor IIF (TFIIS)