GPI-anchor transamidase; GPI transamidase; GPI8 homolog; hGPI8; phosphatidylinositol-glycan biosynthesis class K protein; PIG-K (PIGK, GPI8)

Function: - mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI - during this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with the substrate protein - glycolipid biosynthesis; GPI-anchor biosynthesis - the disulfide bond between PIGK/GPI8 & PIGT is important for normal enzyme activity - forms a complex with PIGT, PIGS, PIGU & GAA1 Structure: - belongs to the peptidase C13 family Compartment: - endoplasmic reticulum membrane - single-pass type 1 membrane protein

General

cysteine proteinase; thiol proteinase membrane protein phosphatidylinositol-glycan biosynthesis protein (PIG-protein)

Properties

SIZE: entity length = 395 aa MW = 45 kD COMPARTMENT: endoplasmic reticulum MOTIF: signal sequence {1-27} cysteine residue {C92} MODIFICATION: cysteine residue {C182 IN PIGT} histidine residue {H164} cysteine residue {C206} Essential for GPI attachment {311-321} transmembrane domain {368-388}

Database Correlations

OMIM 605087 UniProt Q92643 Pfam PF01650 Entrez Gene 10026 Kegg hsa:10026

References

UniProt :accession Q92643