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glyceraldehyde-3-phosphate dehydrogenase (GAPDH, GAPD, CDABP0047, OK/SW-cl12)
Function:
- role in gluconeogenesis & the glycolytic pathway
- role in membrane trafficking in the early secretory pathway
- appears to mediate apoptosis
- cytotoxic stimuli lead to binding of GAPDH to Siah1 via release of nitric oxide.
- the GAPDH-Siah1 complex translocates from cytoplasm to nucleus in a process ultimately leading to cell death [1]
- binds PRKCI
Structure:
- homotetramer.
- belongs to the glyceraldehyde-3-phosphate dehydrogenase family
Compartment:
- cytoplasm,
- postnuclear & perinuclear regions
- membrane
Interactions
molecular events
Related
gluconeogenesis
glycolytic pathway (glycolysis, Embden Myerhof pathway)
Specific
testis glyceraldehyde-3-phosphate dehydrogenase (GAPDHS, GAPDH-2, GAPD2, GAPDH2, GAPDS, HSD35, HSD-35)
General
aldehyde oxidoreductase
dehydrogenase
Properties
SIZE: entity length = 335 aa
MW = 36 kD
COMPARTMENT: cytoplasm
cell nucleus
MOTIF: cofactor-binding site [13-14]
COFACTOR-BOUND: NAD
cofactor-binding site [35-35]
COFACTOR-BOUND: NAD
Tyr phosphorylation site {Y42}
cofactor-binding site [80-80]
COFACTOR-BOUND: NAD
Ser phosphorylation site {S83}
Glyceraldehyde 3-phosphate binding {151-153}
MOTIF: cysteine residue {C152}
Activates thiol group during catalysis {179-179}
binding site
SITE: 182-182
FOR-BINDING-OF: Glyceraldehyde 3-phosphate
Glyceraldehyde 3-phosphate binding {211-212}
MOTIF: Thr phosphorylation site {T211}
binding site
SITE: 234-234
FOR-BINDING-OF: Glyceraldehyde 3-phosphate
cofactor-binding site [316-316]
COFACTOR-BOUND: NAD
Database Correlations
OMIM 138400
UniProt P04406
PFAM correlations
Kegg hsa:2597
ENZYME 1.2.1.12
References
- Sedlak TW, Snyder SH.
Messenger molecules and cell death: therapeutic implications.
JAMA. 2006 Jan 4;295(1):81-9.
PMID: 16391220
- UniProt :accession P04406
- Wikipedia, glyceraldehyde 3-phosphate dehydrogenase entry
http://en.wikipedia.org/wiki/glyceraldehyde_3-phosphate_dehydrogenase