ADP-ribosylation factor-binding protein GGA2; gamma-adaptin-related protein 2; Golgi-localized, gamma ear-containing, ARF-binding protein 2; VHS domain & ear domain of gamma-adaptin; Vear (GGA2, KIAA1080)

Function: - plays a role in protein sorting & trafficking between the trans-Golgi network (TGN) & endosomes - mediates the ARF-dependent recruitment of clathrin to the TGN & binds ubiquitinated proteins & membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif - ubiquitinated (putative) - interacts with NECAP1, TSG101, UBC & AFTPH/aftiphilin - interacts with CNST (putative) - interacts with GGA1 & GGA3 - binds to clathrin & activated ARFs - binds RABEP1 & RABGEF1 - interacts with the type-1 membrane proteins SORT1, SORL1, LRP3, M6PR/CD-MPR, IGF2R/CI-MPR & BACE1 - binds the accessory proteins CCDC91, P200, SYNRG, EPN4 & NECAP2 Structure: - monomer - the VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by 2 Leu (AC-LL motif) - the GAT domain a) responsible for interaction with ARF- GTP, UBC & RABEP1 b) required for recruitment to the TGN c) prevents ARF-GTP hydrolysis - the unstructured hinge region contains clathrin-binding but no autoinhibitory (AC-LL) motifs - the GAE domain binds accessory proteins regulating GGAs function - belongs to the GGA protein family - contains 1 GAE domain - contains 1 GAT domain - contains 1 VHS domain Compartment: - Golgi, trans-Golgi network - membrane; peripheral membrane, endosome membrane Expression: ubiquitously expressed

Related

ADP-ribosylation factor [ARF] subfamily protein gamma adaptin (adaptor-related protein complex, gamma subunit)

Properties

SIZE: entity length = 613 aa MW = 67 kD COMPARTMENT: golgi MOTIF: VHS domain {33-163} GAT {188-315} Unstructured hinge {316-483} GAE {484-605}

Database Correlations

OMIM 606005 UniProt Q9UJY4 PFAM correlations Entrez Gene 23062 Kegg hsa:23062

References

UniProt :accession Q9UJY4