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cytosolic beta-glucosidase; cytosolic beta-glucosidase-like protein 1 (GBA3, CBG, CBGL1)
Function:
1) glycosidase
2) role in intestinal absorption & metabolism of dietary flavonoid glycosides
3) hydrolyzes variety of glycosides including phytoestrogens, flavonols, flavones, flavanones & cyanogens
4) beta-glycosylceramidase activity
- may be involved in a nonlysosomal catabolic pathway of glycosylceramide
5) hydrolysis of terminal, non-reducing beta-D-glucose residues with release of beta-D-glucose
Inhibition:
- inhibited by
- 2,4-dinitrophenyl-2-fluoro-2-deoxy-beta-D-glucopyranoside
- sodium taurocholate
Kinetic parameters:
- KM=10 uM for luteolin-4'-glucoside;
- KM=50 uM for luteolin-7-glucoside;
- Vmax=1.30 umol/min/mg enzyme toward luteolin-4'-glucoside;
- Vmax=2.85 umol/min/mg enzyme toward luteolin-7-glucoside;
- Optimum pH is 6.5, active from pH 5 to 7.5
Structure:
- belongs to the glycosyl hydrolase 1 family, Klotho subfamily
- N-terminus is blocked
Compartment: cytoplasm, cytosol
Alternative splicing: named isoforms=2
Expression:
- expressed in liver, small intestine, colon, spleen & kidney
Pathology:
- down-regulated in
- renal cell carcinomas
- hepatocellular carcinomas
General
glucosidase
human longevity protein
Properties
SIZE: entity length = 469 aa
MW = 54 kD
COMPARTMENT: cytoplasm
MOTIF: binding site
SITE: 17-17
FOR-BINDING-OF: Substrate
binding site
SITE: 120-120
FOR-BINDING-OF: Substrate
binding site
SITE: 164-164
FOR-BINDING-OF: Substrate
glutamate residue {E165}
active site
binding site
SITE: 309-309
FOR-BINDING-OF: Substrate
glutamate residue {E373}
binding site
SITE: 417-417
FOR-BINDING-OF: Substrate
Substrate binding {424-425}
Database Correlations
OMIM 606619
UniProt Q9H227
Pfam PF00232
Entrez Gene 57733
Kegg hsa:57733
ENZYME 3.2.1.21
References
- UniProt :accession Q9H227
- UniProt PubMed refs
- PMID: 17595169
- PMID: 11784319
- PMID: 12594539