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fluorescent treponemal antibody absorption test (FTA, FTA-ABS)

Indications: - syphilis Principle: - treponemal serologic tests used in the diagnosis of syphilis include: - fluorescent treponemal antibody absorption test (FTA) - microhemagglutination Treponema pallidum test (MHA-TP) - TP-PA indirect fluorescent antibody - these tests are based upon detection of treponemal antigens Clinical significance: Sensitivity (serum): - primary: 70-100% - secondary: 100% - tertiary: 96% Methods: - in the FTA, heated serum is mixed with a sonicate of non-pathogenic Reiter treponemes to remove non-specific antibodies - it is placed on a microscope slide to which Treponema pallidum is fixed - slides are incubated, stained with fluorescein-labeled anti-human globulin & viewed by fluorescence microscopy Notes: - for Loincs, see Treponema pallidum Ab in Serum by Immunofluorescence

Related

syphilis

Specific

fluorescent treponemal IgG absorption test fluorescent treponemal IgM absorption test

General

treponemal serologic test for syphilis

References

  1. Clinical Diagnosis & Management by Laboratory Methods, 19th edition, J.B. Henry (ed), W.B. Saunders Co., Philadelphia, PA. 1996, pg 1186
  2. T.Pallidum Ab, IgG (FTA-ABS) Laboratory Test Directory ARUP: 50477