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flame emission spectroscopy (FES)

Principle: - when a metallic salt is heated in a flame, the energy absorbed as heat excites one or more electrons into an excited state - when the excited electrons return to the ground state, electromagnetic radiation is emitted. The wavelength of the radiation emitted are characteristic of the atom/ion under consideration - each metal has its own characteristic emission spectra showing emission of radiation at characteristic wavelenths

General

spectrophotometry

References

  1. Clinical Guide to Laboratory Tests, 3rd ed. Teitz ed., W.B. Saunders, 1995
  2. Clinical Diagnosis & Management by Laboratory Methods, 19th edition, J.B. Henry (ed), W.B. Saunders Co., Philadelphia, PA. 1996, pg 57