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flap structure-specific endonuclease 1; FEN1 endonuclease; maturation factor 1; rad2 homolog (FEN1, MF1, RAD2)

Function: - endonuclease - removes 5' overhanging flaps in DNA repair & processes 5' ends of Okazaki fragments in lagging strands in DNA synthesis - possesses 5' to 3' exonuclease activity on niked or gapped dsDNA - exhibits Rnase H activity - involved in base excision repair - interacts with PCNA - the C-terminal domain binds EP300 - can bind simultaneously to both PCNA & EP300 - acetylated by EP300 a) acetylation inhibits both endonuclease & exonuclease activity b) acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300 Structure: - belongs to the XPG/RAD2 endonuclease family, FEN1 subfamily Compartment: nucleus

Interactions

molecular events

General

endodeoxyribonuclease

Properties

SIZE: entity length = 380 aa MW = 43 kD COMPARTMENT: cell nucleus MOTIF: N {1-104} MOTIF: aspartate residue {D34} DNA-binding motif SITE: 47-47 DNA-binding motif SITE: 70-70 aspartate residue {D86} I {122-253} MOTIF: DNA-binding motif SITE: 158-158 aspartate residue {D181} DNA-binding motif SITE: 231-231 DNA-binding motif SITE: 233-233 PCNA interaction {328-355}

References

  1. UniProt :accession P39748
  2. NIEHS-SNPs http://egp.gs.washington.edu/data/fen1/

Component-of

molecular complex

Databases & Figures

OMIM 600393 UniProt P39748 PFAM correlations Entrez Gene 2237 Kegg hsa:2237 Mechanism of base excision repair (v.2)