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exon junction complex (EJC)
Function:
- dynamic structure consisting of a few core proteins & several more peripheral nuclear & cytoplasmic associated factors that join the complex only transiently either during assembly or during subsequent mRNA metabolism
- core components that remain bound to spliced mRNAs throughout all stages of mRNA metabolism, function to mark the position of the exon-exon junction in the mature mRNA & thus influence downstream processes of gene expression including
a) mRNA splicing
b) nuclear mRNA export
c) subcellular mRNA localization
d) translation efficiency
e) nonsense-mediated mRNA decay (NMD)
- remains associated with mRNAs in the cytoplasm until the mRNAs engage the translation machinery
- its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs
- associates preferentially with mRNAs produced by splicing
- does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs
- associates with both nuclear mRNAs & newly exported cytoplasmic mRNAs
Structure:
- subunits: NCBP1, NCBP2, RNPS1, RBM8A, SRRM1, NXF1, NFX1, RENT1, RENT2, RENT3A, RENT3B, THOC4, MAGOH, CASC3, EIF4A3, ACIN1, DEK, EIF4A3, PNN, SAP18
Compartment: translocates from nucleus to cytoplasm
General
molecular complex
Properties
SUBUNITS: protein mago nashi homolog
COMPARTMENT: cell nucleus
MOTIF: RNA-binding motif
FOR-BINDING-OF: ribonucleic acid
80 kD nuclear cap binding protein
COMPARTMENT: cell nucleus
MOTIF: nuclear translocation signal {3-20}
Thr phosphorylation site {T21}
Ser phosphorylation site {S22}
MIF4G {28-240}
coiled coil {643-713}
MOTIF: Ser phosphorylation site {S674}
nuclear cap binding protein subunit 2
COMPARTMENT: cell nucleus
MOTIF: Ser phosphorylation site {S13}
Ser phosphorylation site {S18}
RNP motif
NAME: RNP motif
SITE: 40-118
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
NAME: ribonucleoprotein-1 motif
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
ribonucleoprotein-2 motif
ribonucleoprotein-2 motif
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
ribonucleoprotein-2 motif
RNA-binding protein with serine-rich domain 1
COMPARTMENT: cytoplasm
cell nucleus
MOTIF: domain {1-220}
MOTIF: domain {1-161}
lysine-rich region {7-64}
MOTIF: lysine residue (SEVERAL)
Ser phosphorylation site {S27}
Ser phosphorylation site {S53}
serine-rich region {67-141}
MOTIF: serine residue (SEVERAL)
domain {69-121}
Ser phosphorylation site {S125}
arginine-rich region {128-154}
MOTIF: arginine residue (SEVERAL)
Ser phosphorylation site {S155}
domain {156-242}
MOTIF: Ser phosphorylation site {S157}
RNP motif
NAME: RNP motif
SITE: 161-240
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
NAME: ribonucleoprotein-1 motif
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
ribonucleoprotein-2 motif
ribonucleoprotein-2 motif
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
ribonucleoprotein-2 motif
Tyr phosphorylation site {Y205}
domain {238-305}
MOTIF: proline-rich region
SITE: 241-298
MOTIF: proline residue (SEVERAL)
Ser phosphorylation site {S251}
Ser phosphorylation site {S266}
Ser phosphorylation site {S287}
Ser phosphorylation site {S289}
RNA-binding protein 8A
COMPARTMENT: cytoplasm
cell nucleus
MOTIF: acetylation site
SITE: N-TERMINUS
EFFECTOR-BOUND: acetyl
Ser phosphorylation site {S42}
Ser phosphorylation site {S46}
Ser phosphorylation site {S56}
RNP motif
NAME: RNP motif
SITE: 73-151
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
NAME: ribonucleoprotein-1 motif
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
ribonucleoprotein-2 motif
ribonucleoprotein-2 motif
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
ribonucleoprotein-2 motif
Ser phosphorylation site {S166}
Ser phosphorylation site {S168}
serine/arginine repetitive matrix protein 1
COMPARTMENT: cell nucleus
MOTIF: domain {1-156}
MOTIF: binding site
SITE: 1-151
FOR-BINDING-OF: deoxyribonucleic acid (DNA)
RNA
Ser phosphorylation site {S10}
PWI {45-118}
arginine-rich region {163-726}
MOTIF: arginine residue (SEVERAL)
proline-rich region
SITE: 210-417
MOTIF: proline residue (SEVERAL)
Ser phosphorylation site {S211}
Thr phosphorylation site {T220}
serine-rich region {278-756}
MOTIF: Ser phosphorylation site (11) {389-549}
domain {300-688}
proline-rich region
SITE: 550-799
MOTIF: proline residue (SEVERAL)
Ser phosphorylation site (10) {560-616}
Ser phosphorylation site (10) {626-775}
Ser phosphorylation site {S781}
lysine-rich region {809-835}
MOTIF: lysine residue (SEVERAL)
alanine-rich region {836-865}
MOTIF: alanine residue (SEVERAL)
nuclear RNA export factor 1
COMPARTMENT: cytoplasm
cell nucleus
MOTIF: ALYREF/THOC4 interaction {2-371}
acetylation site
SITE: N-TERMINUS
EFFECTOR-BOUND: acetyl
nuclear translocation signal {67-100}
nuclear export signal {83-110}
RNP motif
NAME: RNP motif
SITE: 119-198
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
NAME: ribonucleoprotein-1 motif
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
ribonucleoprotein-2 motif
ribonucleoprotein-2 motif
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
ribonucleoprotein-2 motif
leucine-rich repeat
SITE: 266-291
MOTIF: leucine residue (SEVERAL)
leucine-rich repeat
SITE: 292-315
MOTIF: leucine residue (SEVERAL)
leucine-rich repeat
SITE: 316-343
MOTIF: leucine residue (SEVERAL)
leucine-rich repeat
SITE: 344-371
MOTIF: leucine residue (SEVERAL)
NTF2 {386-536}
proline-rich region
SITE: 551-561
MOTIF: proline residue (SEVERAL)
TAP-C {565-619}
transcriptional repressor NF-X1
COMPARTMENT: cell nucleus
MOTIF: PABPC1 and PABC4 interaction {9-26}
Ser phosphorylation site {S49}
Ser phosphorylation site {S50}
Ser phosphorylation site {S90}
RING-finger {358-409}
EFFECTOR-BOUND: Zn+2
FOR-BINDING-OF: DNA motif
Zinc finger
NAME: Zinc finger
SITE: 453-471
EFFECTOR-BOUND: Zn+2
Zinc finger
NAME: Zinc finger
SITE: 506-525
EFFECTOR-BOUND: Zn+2
Zinc finger
NAME: Zinc finger
SITE: 567-586
EFFECTOR-BOUND: Zn+2
Zinc finger
NAME: Zinc finger
SITE: 632-655
EFFECTOR-BOUND: Zn+2
Zinc finger
NAME: Zinc finger
SITE: 694-713
EFFECTOR-BOUND: Zn+2
Zinc finger
NAME: Zinc finger
SITE: 721-740
EFFECTOR-BOUND: Zn+2
Zinc finger
NAME: Zinc finger
SITE: 832-854
EFFECTOR-BOUND: Zn+2
Zinc finger
NAME: Zinc finger
SITE: 863-884
EFFECTOR-BOUND: Zn+2
R3H {994-1062}
MOTIF: Tyr phosphorylation site {Y1041}
proline-rich region
SITE: 1084-1089
MOTIF: proline residue (SEVERAL)
regulator of nonsense transcripts 1
COMPARTMENT: cytoplasm
MOTIF: Sufficient for interaction with RENT2 {1-415}
MOTIF: proline-rich region
SITE: 47-80
MOTIF: proline residue (SEVERAL)
Zn finger C2H2-type
SITE: 131-159
EFFECTOR-BOUND: Zn+2
Zn finger C4-type
SITE: 183-213
EFFECTOR-BOUND: Zn+2
ATP-binding site
NAME: ATP-binding site
SITE: 503-510
serine-rich region {1042-1129}
MOTIF: serine residue (SEVERAL)
Q motif {1089-1090}
Ser phosphorylation site {S1089}
Q motif {1107-1108}
Ser phosphorylation site {S1107}
Ser phosphorylation site {S1127}
regulator of nonsense transcripts 2
COMPARTMENT: cytoplasm
cell nucleus
MOTIF: lysine-rich region {6-136}
MOTIF: lysine residue (SEVERAL)
coiled coil {54-134}
binding site
SITE: 94-133
FOR-BINDING-OF: regulator of nonsense transcripts 1
MIF4G 1 {168-431}
coiled coil {487-559}
MIF4G 2 {569-758}
binding site
SITE: 711-928
FOR-BINDING-OF: regulator of nonsense transcripts 3A
regulator of nonsense transcripts 3B
binding site
SITE: 757-1272
FOR-BINDING-OF: eukaryotic initiation factor 4A1
eukaryotic translation initiation factor 1
MOTIF: MIF4G 3 {773-986}
binding site
SITE: 839-859
FOR-BINDING-OF: regulator of nonsense transcripts 3B
glutamate-rich region {1025-1094}
MOTIF: glutamate residue (SEVERAL)
binding site
SITE: 1084-1272
FOR-BINDING-OF: regulator of nonsense transcripts 1
regulator of nonsense transcripts 3A
COMPARTMENT: cytoplasm
cell nucleus
MOTIF: Ser phosphorylation site {S68}
regulator of nonsense transcripts 3B
COMPARTMENT: cytoplasm
cell nucleus
MOTIF: binding site
SITE: 30-255
FOR-BINDING-OF: regulator of nonsense transcripts 2
MOTIF: binding site
SITE: 52-57
FOR-BINDING-OF: regulator of nonsense transcripts 2
binding site
SITE: 430-447
FOR-BINDING-OF: RNA-binding protein 8A
NMD
THO complex subunit 4
COMPARTMENT: cell nucleus
MOTIF: acetylation site
SITE: 1-1
EFFECTOR-BOUND: acetyl
glycine-rich region {20-230}
MOTIF: RRM {105-182}
protein CASC3
COMPARTMENT: cytoplasm
cell nucleus
MOTIF: Ser phosphorylation site {S37}
glycine-rich region {41-46}
MOTIF: Ser phosphorylation site {S45}
coiled coil {95-131}
binding site
SITE: 137-283
FOR-BINDING-OF: ribonucleic acid
MOTIF: EJC {137-283}
Ser phosphorylation site {S148}
nuclear translocation signal {204-210}
nuclear translocation signal {254-262}
Ser phosphorylation site {S363}
Ser phosphorylation site {S373}
cytoplasmic stress granules {377-703}
MOTIF: proline-rich region
SITE: 392-395
MOTIF: proline residue (SEVERAL)
proline-rich region
SITE: 425-428
MOTIF: proline residue (SEVERAL)
nuclear export signal {462-466}
Ser phosphorylation site {S477}
proline-rich region
SITE: 643-648
MOTIF: proline residue (SEVERAL)
proline-rich region
SITE: 692-695
MOTIF: proline residue (SEVERAL)
eukaryotic initiation factor 4A-like
COMPARTMENT: cell nucleus
MOTIF: DNA-binding motif
active site
MOTIF: P-loop
FOR-BINDING-OF: adenosine triphosphate
RNA-binding motif
FOR-BINDING-OF: ribonucleic acid
apoptotic chromatin condensation inducer in the nucleus
COMPARTMENT: cell nucleus
MOTIF: SAP {72-106}
glutamate-rich region {142-442}
MOTIF: glutamate residue (SEVERAL)
Ser phosphorylation site {S166}
Ser phosphorylation site {S169}
Ser phosphorylation site {S208}
Ser phosphorylation site {S216}
Ser phosphorylation site {S228}
Ser phosphorylation site {S229}
Ser phosphorylation site {S240}
Ser phosphorylation site {S243}
Thr phosphorylation site {T254}
Ser phosphorylation site {S256}
Thr phosphorylation site {T269}
Ser phosphorylation site {S295}
Ser phosphorylation site {S328}
Ser phosphorylation site {S365}
Ser phosphorylation site {S384}
Ser phosphorylation site {S386}
Ser phosphorylation site {S388}
Thr phosphorylation site {T393}
Ser phosphorylation site {S400}
Thr phosphorylation site {T408}
Ser phosphorylation site {S410}
Thr phosphorylation site {T414}
Ser phosphorylation site {S424}
Ser phosphorylation site {S425}
Ser phosphorylation site {S434}
Ser phosphorylation site {S453}
Ser phosphorylation site {S478}
Ser phosphorylation site {S490}
Tyr phosphorylation site {Y512}
Ser phosphorylation site {S561}
serine-rich region {573-676}
MOTIF: serine residue (SEVERAL)
Ser phosphorylation site {S655}
Ser phosphorylation site {S657}
Ser phosphorylation site {S661}
Ser phosphorylation site {S664}
Ser phosphorylation site {S675}
Ser phosphorylation site {S676}
Thr phosphorylation site {T682}
Ser phosphorylation site {S710}
Ser phosphorylation site {S714}
Thr phosphorylation site {T720}
Ser phosphorylation site {S729}
Ser phosphorylation site {S814}
Ser phosphorylation site {S815}
Ser phosphorylation site {S825}
Thr phosphorylation site {T830}
Ser phosphorylation site {S838}
Thr phosphorylation site {T840}
Ser phosphorylation site {S895}
Ser phosphorylation site {S898}
Thr phosphorylation site {T976}
Thr phosphorylation site {T978}
Ser phosphorylation site {S981}
Ser phosphorylation site {S983}
Ser phosphorylation site {S987}
Ser phosphorylation site {S990}
Ser phosphorylation site {S1004}
proteolytic site {1093-1094}
proline-rich region
SITE: 1114-1131
MOTIF: proline residue (SEVERAL)
lysine-rich region {1132-1341}
MOTIF: lysine residue (SEVERAL)
Ser phosphorylation site {S1176}
Ser phosphorylation site {S1178}
Ser phosphorylation site {S1180}
Ser phosphorylation site {S1284}
Ser phosphorylation site {S1329}
Ser phosphorylation site {S1331}
Thr phosphorylation site {T1332}
protein DEK
COMPARTMENT: cell nucleus
MOTIF: acetylation site
SITE: N-TERMINUS
EFFECTOR-BOUND: acetyl
acidic region {30-49}
MOTIF: acidic residue (SEVERAL)
Ser phosphorylation site {S32}
Ser phosphorylation site {S51}
SAP {149-183}
nuclear translocation signal {205-221}
Ser phosphorylation site {S227}
acidic region {228-236}
MOTIF: acidic residue (SEVERAL)
Ser phosphorylation site {S230}
Ser phosphorylation site {S231}
Ser phosphorylation site {S232}
acidic region {241-254}
MOTIF: acidic residue (SEVERAL)
Ser phosphorylation site {S243}
Ser phosphorylation site {S244}
Ser phosphorylation site {S251}
Ser phosphorylation site {S276}
acidic region {300-310}
MOTIF: acidic residue (SEVERAL)
Ser phosphorylation site {S301}
Ser phosphorylation site {S303}
Ser phosphorylation site {S306}
Ser phosphorylation site {S307}
DNA-binding motif
SITE: 337-351
DNA-binding motif
SITE: 367-371
eukaryotic initiation factor 4A-like
COMPARTMENT: cell nucleus
MOTIF: DNA-binding motif
active site
MOTIF: P-loop
FOR-BINDING-OF: adenosine triphosphate
RNA-binding motif
FOR-BINDING-OF: ribonucleic acid
pinin
COMPARTMENT: cytoplasm
cell nucleus
MOTIF: binding site
SITE: 1-283
FOR-BINDING-OF: RNA-binding protein with serine-rich domain 1
MOTIF: 5' splicing {1-166}
binding site
SITE: 1-97
FOR-BINDING-OF: keratin, type II cytoskeletal 8
keratin, type I cytoskeletal 18
keratin, type I cytoskeletal 19
coiled coil {1-31}
acetylation site
SITE: 1-1
EFFECTOR-BOUND: acetyl
Ser phosphorylation site {S65}
coiled coil {162-233}
glutamate-rich region {171-470}
MOTIF: glutamate residue (SEVERAL)
coiled coil {286-378}
Ser phosphorylation site {S346}
Ser phosphorylation site {S442}
coiled coil {445-466}
glutamine-rich region {468-519}
MOTIF: glutamine residue (SEVERAL)
serine-rich region {551-703}
MOTIF: serine residue (SEVERAL)
binding site
SITE: 605-716
FOR-BINDING-OF: cyclophilin G
MOTIF: arginine-rich region {631-716}
MOTIF: arginine residue (SEVERAL)
SAP18 protein
References
UniProt :accession Q9BZI7
Components
80 kD nuclear cap binding protein (NCBP 80 kD subunit, nuclear cap-binding protein subunit 1, CBP80, NCBP1, NCBP)
apoptotic chromatin condensation inducer in the nucleus; acinus (ACIN1, ACINUS, KIAA0670)
eukaryotic translation initiation factor 4A-like; NUK-34 (EIF4A3)
nuclear cap binding protein subunit 2 (20 kD nuclear cap binding protein, NCBP 20 kD subunit, CBP20, NCBP interacting protein 1, NIP1)
nuclear mRNA cap-binding protein
nuclear RNA export factor 1; Tip-associated protein; Tip-associating protein; mRNA export factor TAP (NXF1, TAP)
pinin (140 kD nuclear & cell adhesion-related phosphoprotein, domain-rich serine protein, DRS-protein, DRSP, melanoma metastasis clone A protein, desmosome-associated protein, SR-like protein, nuclear protein SDK3, PNN, DRS, MEMA)
protein CASC3; cancer susceptibility candidate gene 3 protein; metastatic lymph node gene 51 protein; MLN 51; protein barentsz; Btz (CASC3 MLN51)
protein DEK (DEK)
protein mago nashi homolog (MAGOH)
regulator of nonsense transcripts 1 (nonsense mRNA reducing factor 1, NORF1, up-frameshift suppressor 1 homolog, hUpf1, RENT1, KIAA0221, UPF1)
regulator of nonsense transcripts 2 (nonsense mRNA reducing factor 2, up-frameshift suppressor 2 homolog, hUpf2, UPF2, KIAA1408, RENT2)
regulator of nonsense transcripts 3A (nonsense mRNA reducing factor 3A, up-frameshift suppressor homolog 3A, hUpf3, UPF3A, RENT3A, UPF3)
regulator of nonsense transcripts 3B (nonsense mRNA reducing factor 3B, up-frameshift suppressor homolog 3B, hUpf3B, hUpf3p-X, UPF3B, RENT3B, UPF3X)
RNA-binding protein 8A; RNA-binding motif protein 8A; ribonucleoprotein RBM8A; RNA-binding protein Y14; binder of OVCA1-1; BOV-1 (RBM8A, RBM8, HSPC114, MDS014)
RNA-binding protein with serine-rich domain 1; SR-related protein LDC2 (RNPS1, LDC2)
serine/arginine repetitive matrix protein 1; SR-related nuclear matrix protein of 160 kD; SRm160; Ser/Arg-related nuclear matrix protein (SRRM1 SRM160)
sin3-associated peptide, 18kD (histone deacetylase complex subunit SAP18, SAP18)
THO complex subunit 4; Tho4; Ally of AML-1 & LEF-1; transcriptional coactivator Aly/REF; bZIP-enhancing factor BEF (THOC4, ALY, BEF, ALYREF)
THO/TREX complex
transcriptional repressor NF-X1; nuclear transcription factor, X box-binding protein 1 (NFX1, NFX2)