telomerase-binding protein EST1A; ever shorter telomeres 1A; telomerase subunit EST1A (EST1A, SMG6, C17orf31, KIAA0732)

Function: - component of the telomerase ribonucleoprotein complex - promotes in vitro the ability of TERT to elongate telomeres - overexpression induces telomere uncapping, chromosomal end-to-end fusions (telomeric DNA persists at fusion points) & did not perturb TRF2 telomeric localization - dephosphorylates RENT1 - plays a role in nonsense-mediated mRNA decay - may function as endonuclease - degrades single-stranded RNA (ssRNA), but not ssDNA or dsRNA - interacts with TERT, independently of the telomerase RNA - binds to the single-stranded 5'-(GTGTGG)(4)GTGT-3' telomeric DNA, but not to a telomerase RNA template component (TER) - interacts with PP2A catalytic subunits, SMG1, RENT1, RENT2 & RENT3B Cofactor: Mn+2 Compartment: - nucleus, nucleolus - particularly enriched in the nucleolus Alternative splicing: named isoforms=2 Expression: ubiquitous

Properties

SIZE: entity length = 1419 aa MW = 160 kD COMPARTMENT: cell nucleus MOTIF: telomeric DNA interaction {114-503} MOTIF: coiled coil {163-193} coiled coil {567-625} coiled coil {1197-1239} manganese [Mn]-binding site SITE: 1251-1251 manganese [Mn]-binding site SITE: 1353-1353 manganese [Mn]-binding site SITE: 1392-1392

References

UniProt :accession Q86US8