DNA excision repair protein ERCC-6-like ( ATP-dependent helicase ERCC6-like, PLK1-interacting checkpoint helicase, tumor antigen BJ-HCC-15) (ERCC6L PICH)

Function: - DNA helicase - acts as an essential component of the spindle assembly checkpoint - contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores & monitoring tension on centromeric chromatin - acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase - interacts with PLK1, which phosphorylates it; both proteins are mutually dependent on each other for correct subcellular localization; phosphorylation by PLK1 prevents the association with chromosome arms & restricts its localization to the kinetochore-centromere region Structure: - belongs to the SNF2/RAD54 helicase family - contains 1 helicase ATP-binding domain - contains 1 helicase C-terminal domain - contains 2 TPR repeats Compartment: - centromere - localizes to kinetochores, inner centromeres & thin threads connecting separating chromosomes even during anaphase - in prometaphase cells, it mostly concentrates in between kinetochores - in metaphase, it localizes to numerous thin threads that stretch between sister kinetochores of the aligned chromosomes - evolution from inner centromeres to thin threads takes place in response to tension - resolution of thin threads requires topoisomerase 2-alpha (TOP2A) after anaphase onset

General

helicase (DNA unwinding protein, DNA untwisting protein) phosphoprotein

Properties

SIZE: entity length = 1250 aa MW = 141 kD COMPARTMENT: cell nucleus MOTIF: tetratricopeptide repeat {21-54} helicase NAME: helicase SITE: 109-277 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 122-129 DEAD/H box NAME: DEAD/H box SITE: 228-231 Helicase C-terminal {464-620} Ser phosphorylation site {S1021} Thr phosphorylation site {T1063} tetratricopeptide repeat {1200-1233}

Database Correlations

UniProt Q2NKX8 PFAM correlations Kegg hsa:5482

References

UniProt :accession Q2NKX8